Original title: Metody stanovení obsahu polyhydroxyalkanoátů v bakteriálních buňkách
Translated title: Analytical methods for determination of polyhydroxyalcanoates content in bacterial cells
Authors: Černayová, Diana ; Enev, Vojtěch (referee) ; Sedláček, Petr (advisor)
Document type: Bachelor's theses
Year: 2018
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [cze] [eng]
Táto bakalárska práca sa zaoberá metódami na stanovenie polyhydroxyalkanoátov (PHA) v bunkách baktérie Cupriavidus necator H16. Medzi použité metódy patrí infračervnená spektroskopia s Fourierovou transformáciou (FTIR), Ramanova spektroskopia, turbidimetria a termické analýzy (termogravimetrická analýza a diferenčná skenovacia kalorimetria). Výsledky z metód boli porovnávané s obsahom PHA stanoveného plynovou chromatografiou. Pri infračervenej spektroskopií s Fourierovou transformáciou a Ramanovej spektroskopií sa zistila pozitívna korelácia pomeru charakteristických pásov k obsahu PHA. S použitím FTIR bolo možné okrem kvantitatívneho stanovenia PHA zistiť aj kvalitu vzorky (kryštalickosť /amorfnosť). Ďalej sa použila turbidimetrická analýza, pri ktorej sa sledovala závislosť rozptylu UV-VIS žiarenia na obsahu PHA v bakteriálnych bunkách. Najefektívnejšou technikou sa ukázala byť termogravimetrická analýza. Závislosť úbytku hmotnosti v oblasti spaľovania PHA, na jeho obsahu (zisteného plynovou chromatografiou) bola stanovená s najvyššou koreláciou k plynovej chromatografií. Metóda DSC sa neukázala byť vhodnou alternatívnou náhradou na stanovenie obsahu PHA, ale umožnila stanoviť či sa polymér nachádza v stave kryštalickom, alebo amorfnom. This bachelor thesis deals with methods that are used for determination of polyhydroxyalkanoates (PHA) in cells of bacteria Cupriavidus necator H16. To the applied methods belong Fourier transform infrared spectroscopy, Raman spectoscopy, turbidimetry and thermal analysis (thermogravimetric analysis and differential scanning calorimetry). The results of each method were compared to amount of PHA determinated by gas chromatography. Fourier transform infrared spectroscopy and Raman spectroscopy revealed a linear relation between ratio of characteristic peaks and amount of PHA. FTIR enabled to determine not only the quantitative amount of PHA, but the quality of the sample (cristallinity, amorphous state) as well. Another method was turbidimetry, which has shown correlation between dispersion of UV-VIS light to amount of PHA in bacterial cells. Thermogravimetric analysis was the most effective technique for determination of PHA. The dependence of weight loss of PHA on its content (found by gas chromatography) was determined with the highest accuracy to gas chromatography. Tha last method- DSC was not alternative replacement for quantitative PHA determination. However, DSC indicated crystalline and amorphous state of the polymer.
Keywords: Cupriavidus necator H16; FTIR; PHA; Polyhydroxyalkanoates; polyhydroxybutyrate; Raman spectroscopy; thermal analysis; turbidimetry; Cupriavidus necator H16; FTIR; PHA; Polyhydroxyalkanoáty; polyhydroxybutyrát; Ramanova spektoskopia; termická analýza; turbidimetria

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/81436

Permalink: http://www.nusl.cz/ntk/nusl-377546


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses