Original title: Transkripční odpověď ruseníček rolního (Arabidopsis thaliana) na metylační stres
Translated title: Transcriptional response of plants to genotoxic stress and water deficit
Authors: Libus, Jiří ; Štorchová, Helena (advisor) ; Cvrčková, Fatima (referee) ; Kovařík, Aleš (referee)
Document type: Doctoral theses
Year: 2007
Language: eng
Abstract: 7 Conclusions 1) cDNA array was prepared and used to assay transcript abundances of 376 selected Ara- bidopsis transcripts following various treatments with MMS. a) LoTrEC, clustering algorithm based on local trends of expression profiles, was de- signed and applied to the data. It succeeded to discover functionally rellevant clusters of expression profiles. b) Transcriptional responses to various MMS treatment regimes were investigated. While high MMS concentration seemed only to induce nonspecific stress reaction, the low and combined MMS resulted in a set of more specific expression changes. c) Expression levels of five transcripts were estimated by qRT-PCR. Trends of most of the profiles were confirmed. 2) Expression of 3 genes related to drought stress and/or response to cytokinin were mea- sured by qRT-PCR in wild type and ZOG1 transgenic plants. Transcript levels of all the genes were altered by water deficit. a) Although there are no significant macroscopic differences between wild type and ZOG1 transgenic plants, the mRNA abundances appeared to be influenced by the genotype. b) Leaf position (age) significantly influenced the expression of cig1 and ZOG1 driven by SAG12 promoter. c) RT primed with oligo-dT appeared more eficient than random hexanucleotide-primed reaction for 3 out of 5 mRNAs...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/95139

Permalink: http://www.nusl.cz/ntk/nusl-373548


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses