Original title: The use of real time PCR method for evaluation of magnetic microspheres
Authors: Trachtová, Š. ; Rittich, B. ; Horák, Daniel ; Španová, A.
Document type: Papers
Conference/Event: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Year: 2012
Language: eng
Abstract: The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA-based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 mu l microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer.
Keywords: magnetic microspheres; real time PCR
Host item entry: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3

Institution: Institute of Macromolecular Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0279473

Permalink: http://www.nusl.cz/ntk/nusl-371060


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Research > Institutes ASCR > Institute of Macromolecular Chemistry
Conference materials > Papers