Proteome dynamics during seed germination

Habánová, Hana

Seed germination is a crucial phase of the plant life cycle. Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination are far from being resolved. Most of the early events of germination are mediated by molecules stored in seeds during the seed development and thus we employed proteomic analyses to elucidate the mechanism behind this process. This thesis - Proteome dynamics during seed germination - reviews present-day knowledge of this topic. In the experimental part, the results of three related research projects are summarized. First, the effects of a novel class of growth regulators were analyzed in the barley germination assay and the optimal concentrations were determined. The methodology for an improved analysis of barley seed proteins was developed and the optimized targeted analysis will allows the detection of over 4,000 proteins. Finally, Arabidopsis thaliana seed proteome was analyzed and the results indicate that (i) an alternative MS/MS data processing significantly improves the detection limits and (ii) the proteasome-ubiquitin system plays a crucial role in adjusting the seed proteome dynamics during the germination progress.

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