Charakterizace a regulace fenotypu kmenových rakovinných buněk v buňkách lidského kolorektálního karcinomu

Trávníčková, Jana

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jana Trávníčková Supervisor: Prof. RNDr. Lenka Skálová Specialized supervisor: Dr. Frédéric Hollande Title of diploma thesis: Characterisation and regulation of the cancer stem cells'phenotype in human colorectal cancer cells. Colorectal cancer nowadays represents one of the most common life threatening malignancies worldwide. Well describing and understanding the causes and development of this illness, especially on the molecular basis would provide a progress in the treatment. Defining cancer stem cells (CSC) that are considered as a potential cause of the tumour resistance and relapse would result in the development of a new targeted therapy. CRC1, colorectal cancer cell line recently established by the laboratory IGF in Montpellier was confirmed to possess the primitive morphology and to contain a larger amount of stem-like cells. This cell line is positive of stem-like-cell markers such as CK-18 and EphB2 and its morphology observed in electron microscope was defined as primitive with big nuclei and rare presence of other organelles. Change of Claudin 2 (CLDN2) expression is considered as another potential cause of colorectal cancer. Its increased expression is connected to the...

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