Vnitrodruhová variabilita flebotoma Phlebotomus sergenti, hlavního přenašeče leishmania tropica

Dvořák, Vít

"Intraspecific variability of Phlebotomus sergenti, a major vector of Leishmania tropica" Vít Dvořák PhD. Thesis, defended in September 16, 2008 Phlebotomus sergenti is the main vector of Leishmania tropica, a causative agent of human cutaneous leishmaniasis, therefore a sand fly species of great medical importance. As it has a very broad range of distribution, a notable intraspecific variability was expected and a possibility of several cryptic species was postulated with differences in ecology, host preferences and vectorial capacity, meaning important implications in diagnostics, epidemiology and medical therapy. The presented thesis studied intraspecific variability of P. sergenti by crossmating analysis of laboratory colonies with different geographical origin as well as by several approaches of molecular taxonomy deployed on specimens from wild populations collected in the field. We demonstrated that crossing is possible between P. sergenti laboratory-reared specimens from colonies originating from Turkey and Israel. Succesful mating and insemination was observed, viable hybrid F1 and F2 offsprings were obtained from both parental combinations, no statistically significant difference in the egg production was found in the hybrids. Analysis of wild populations of P. sergenti from Turkey, Israel, Syria,...

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