Original title: Charakterizace membránového proteinu DREPP
Translated title: Characterization of membrane protein DREPP
Authors: Vosolsobě, Stanislav ; Schwarzerová, Kateřina (advisor) ; Cvrčková, Fatima (referee)
Document type: Master’s theses
Year: 2010
Language: cze
Abstract: Proteins of DREPP family (20-25 kDa, syn. PCaP1 in Arabidopsis thaliana) first appeared in ferns and we have shown that several independent duplications of DREPP protein occurred during evolution of large families (Poaceae, Brassicaceae, Solanaceae and Asteraceae) and in group Coniferophyta. Secondary losses of one paralogue occurred in subfamilies Pooideae and Solanoideae.We have also detected two large-scale modification of DREEP protein in Asparagales and Brassicaceae (this divergent paralogue was previously described as MAP18 protein). We have examined colinearity of chromosome fragments in vicinity both PCaP1 and MAP18 paralogues in Arabidopsis thaliana and we hypothesize that MAP18 gene arose during genome duplication on the origin of Brassicaceae family. DREPP protein was previously identified in detergent-resistant membrane microdomain fraction and a myristyl anchor was shown to be necessary for their membrane localization. Membrane association was shown to be modified by the interaction of unique N-terminal domain with PtdInsPs, which was inhibited by binding of Ca-calmodulin (Nagasaki et al., 2008). The mutation of Gly2 by Ala in the myristilation site, or C-terminal GFP-fusion (GFP-DREPP), affect membrane association in Arabidopsis thaliana (Nagasaki et al., 2008). Several DREPP paralogues in...
Keywords: Arabidopsis thaliana; biolistic; developmentally regulated plasma membrane polypeptide; DREPP; GFP fusion; MAP18; microtubul; microtubule-asociated protein; Nicotiana tabacum; PCaP1; PCaP2; plasma membrane; plasma membrane-asociated cation-binding protein; cytoskelet; huseníček; mikrotubuly; plasmatická membrána; tabák

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/31373

Permalink: http://www.nusl.cz/ntk/nusl-295860

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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
 Record created 2017-05-09, last modified 2017-06-26

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