Original title: Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode
Authors: Skalová, Štěpánka ; Stávková, K. ; Vyskočil, V. ; Barek, J.
Document type: Papers
Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year: 2016
Language: eng
Abstract: 2-Nitrofluorene (2-NF) is a nitrated polycyclic aromatic hydrocarbon (NPAH) which occurs as the environmental pollutant. It is a potential carcinogen and mutagen. Interaction of deoxyribonucleic acid (DNA) with 2-NF was monitored using an electrochemical DNA biosensor prepared from a glassy carbon electrode (GCE) and low-molecular-weight DNA by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and square-wave voltammetry (SWV).\nThere were no damaging interactions observed between DNA and 2-NF using EIS. However, CV shows intercalation of this substance into the DNA structure to form the complex 2-NF–DNA. Intercalation was also observed by SWV, confirming intercalation to reduce the number of electroactive sites and thus reducing the peak heights of adenosine and guanosine.
Keywords: DNA biosensor; glassy carbon electrode; voltammetry
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0259939

Permalink: http://www.nusl.cz/ntk/nusl-251395


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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers