Original title: Plasmide DNA Isolation from Bacteria and Transfection to HEK293 Cell Line
Authors: Karmazínová, K.
Document type: Papers
Language: cze
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: Isolation DNA is a one of the basic methods in molecular biology. There are several methods of DNA amplification and isolation. In this paper phenol-chloroform extraction of three plasmid types is used: Channelrhodopsin-2, ASAP and KIR. Seven plasmids were isolated in total. These plasmids are then validated using gel electrophoresis. Successfully isolated plasmids are then transfected to HEK293 and taken on confocal microscope 24 hours after transfection.
Keywords: competent cells; DNA isolation; HEK293; transfection
Host item entry: Proceedings of the 21st Conference STUDENT EEICT 2015, ISBN 978-80-214-5148-3

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/42951

Permalink: http://www.nusl.cz/ntk/nusl-207142


The record appears in these collections:
Universities and colleges > Public universities > Brno University of Technology
Conference materials > Papers