Original title: Electrochemistry as a Tool for an Enzyme Characterization
Authors: Gál, M. ; Krahulec, J. ; Jiríčková, K. ; Sokolová, Romana ; Híveš, J.
Document type: Papers
Conference/Event: Moderní Elektrochemické Metody /34./, Jetřichovice (CZ), 2014-05-19 / 2014-05-23
Year: 2014
Language: eng
Abstract: Basic biochemical properties such as Michaelis constant (KM) and turnover number (kcat) of enzyme enteropeptidase was measured by electrochemical impedance spectroscopy. Both values determined by impedance measurements are in good agreement with those obtained by traditional spectroscopic techniques. Therefore one can suppose that electrochemical methods might be successful for such measurements also in the case where usual ones (UV-Vis, fluorescence spectroscopy) are not able to determine these enzyme characteristics.
Keywords: electrochemistry; Enterokinase; free enzymes; Impedance spectroscopy; spectroscopy; spektroskopie; volné enzymy
Host item entry: Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody, ISBN 978-80-905221-2-1

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234055

Permalink: http://www.nusl.cz/ntk/nusl-173883

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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers
 Record created 2014-06-19, last modified 2017-01-18

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