Original title:
(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer
Authors:
Josypčuk, Bohdan ; Mareček, Vladimír ; Yosypchuk, O. Document type: Papers Conference/Event: Moderní elektrochemické metody /32./, Jetřichovice (CZ), 2012-05-21 / 2012-05-25
Year:
2012
Language:
eng Abstract:
Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 μg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor.
Keywords:
amalgam electrodes; monolayer; voltammetry Project no.: GAP206/11/1638 (CEP), GAP208/12/1645 (CEP), IAA400400806 (CEP) Funding provider: GA ČR, GA ČR, GA AV ČR Host item entry: XXXII. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-0-7
Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0007008