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Vliv kombinace biouhlu a zinečnatých nanočástic na rostliny kukuřice seté kultivovaných v in vitro podmínkách
Szomolaiová, Erika
Combined application of zinc oxide nanoparticles (ZnO NPs) and biochar could be a perspective tool for enhancing plant viability and mitigating a large scale of stresses in crops. To investigate effect of different concentrations of ZnO NPs (0, 50, 100 and 1000 mg/L), biochar, and their combination on corn plants (Zea mays L.), in vitro experiment took place. Zinc uptake in form of zinc NPs and possible effect of biochar on zinc uptake and accumulation were determined by atomic absorption spectrometry. A positive correlation between ZnO NPs concentration and zinc content in shoots was identified. There was no significant difference observed in plants treated with biochar. Physiological state of plants was evaluated by spectrophotometric analysis of photosynthetic pigments and secondary metabolites. The most significant effect was observed in content of phenolic compounds that increased with increasing ZnO NPs concentration. On the contrary, presence of biochar resulted in significant decrease (p < 0,05) of polyphenols.
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GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES
Rössner ml., Pavel ; Vrbová, Kristýna ; Strapáčová, S. ; Rössnerová, Andrea ; Ambrož, Antonín ; Brzicová, Táňa ; Líbalová, Helena ; Javorková, Eliška ; Zajícová, Alena ; Holáň, Vladimír ; Kulich, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Coufalík, Pavel ; Křůmal, Kamil ; Čapka, Lukáš ; Dočekal, Bohumil ; Šerý, Omar ; Machala, M. ; Topinka, Jan
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
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