|
|
Comparison of reagents for the determination of the functional activity of coagulation factors by the coagulation method.
Štorková, Marie ; Sadílek, Petr (advisor) ; Vrbacký, Filip (referee)
This bachelor thesis deals with the introduction of new more sensitive reagents for the determination of plasma coagulation factor activity of the intrinsic pathway into the hematology laboratory. The theoretical part deals with a brief description of the whole hemostasis process, individual coagulation factors, pathologies of hemostasis and determination of functional activity of coagulation factors. The practical part is devoted to a comparative study of the functional activity of coagulation factors VIII, IX, XI and XII. This study compares the results of the individual factors measured with the new STA C.K. Prest 5 reagents in combination with the respective immunodeficient plasmas with the results obtained by methods using the original STA PTT A reagents in combination with classical deficient plasmas. The measured values were then evaluated using the linear regression method and Bland-Altman plot to assess whether the methods using the new reagents could be implemented in the laboratory. The calculated values of the correlation coefficients of the individual methods are higher than r = 0.98, which together with the equations of the lines of the correlation plots indicate a close correlation between the results measured with the original reagents and those measured with the newly introduced...
|
|
| |
|
| |
|
|
Detection CD56 antigenic expression of the patient with morfology report LGL in the peripheral blood.
Procházková, Irena ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
Author: Bc. Irena Procházková Job title: Detekce exprese antigenu CD56 u pacientů s morfologickým nálezem LGL v periferní krvi Diploma work Charles Univerzity in Prague, Faculty of Pharmacy in Hradec Králové, Biological and medical department Field of study: Expert of specialized methods Abstract The goal of this diploma work was gathering evidence to support the theory, that LGL cells, large granular lymphocytes, are identical to the population of NK cells, that are detectable by antigen CD56. Numbers of the CD56+ cells were gained by flow cytometry measuring the expression of antigen CD56 labeled with antibody against CD56. The correlation was reviewed with the numbers of LGL cells, which were found in the periferal blood of the patient by microscopic evaluation with common panoptical staining of blood coat. There were used the absolute counts of detected parameters for this comparison. The leukocytes from the measured blood count of the patients on the analyzer Advia 2120i were used for calculation the absolute counts of LGL cells. In the group of patients with immunodeficiencies and various forms of allergy where established an increased number of CD56+/CD3- cells (NK cells), we performed a correlation with the number of LGL cells defined microscopically. We found a strong correlation betwwen the two...
|
|
|
Morphology and cytochemical diagnosis of hairy cells leukemia
Bergerová, Lenka ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
5 Title: Morphology and cytochemical diagnosis of hairy cells leukemia (Bachelor thesis) Author: Lenka Bergerová Department: Charles University in Prague - Faculty of Pharmacy in Hradec Králové, branch of study Medical Laboratory Technician Supervisor: MUDr. Dana Mikulenková Abstract Hairy cell leukemia is the chronic lymphoproliferative disorder of clonal character arising from mature B cells. In the peripheral blood smear and in the bone marrow smear, we find the typical leukemic cells which are characteristic by their size, morphology of cell nucleus and cytoplasm. They are bigger than healthy lymphocytes, and they have most frequently the kidney shaped nucleus with fine net-like chromatin and the cytoplasm projecting into fine salients all over the cell perimeter. In the blood count we usually find a mild pancytopenia. To demonstrate the presence of the pathological cells in the peripheral blood smear and the bone marrow smear, we use the panoptic staining method according to Pappenheim. Among other examinations contributing to the diagnostics of this disease belongcytochemical examination of acid phosphatase and acid phosphatase resistent for tartarate, immunophenotypic findings and trepan bone marrow biopsy. In addition to the typical form of hairy cell leukemia we distinguish another variant form of...
|
|
| |
|
|
Search for a suitable housekeeping gene for relative quantification in patients with the chronic lymphocytic leukemia
Ryšková, Barbora ; Vrbacký, Filip (advisor) ; Sadílek, Petr (referee)
5 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Ryšková Supervisor: Mgr. Filip Vrbacký Title of diploma thesis: Search for a suitable houskeeping gene for relative guantification in patients with the chronic lymfocytic leukemia Chronic lymphocytic leukemia (CLL) is the most common form of leukemia found in adults and that is why it is studied very intensively. The cause of CLL is still unknown and it is not known how to cure this lyphoproliferative disorder without high risk of complications that may occur with a bone marrow transplantation. Altered gene expression of angiogenic factors in CLL is still insufficiently known too, so we decided to study this topic in our laboratory. Our task was to establish method for quantification of proper housekeeping gene that can be used for normalization of results of quantification of expression of some angiogenic factors by real-time PCR. We decided to use Abl1 gene but that gene is alternatively spliced. Exon 1 containing variant is housekeeping only and we had to take it into account when choosing proper method. I managed to transform Escherichia coli cells and amplify plasmid containing Abl1 sequence. Plasmid was isolated, quantificated by spectrophotometer and used to...
|
|
| |
|
|
Laboratory diagnostics of anemias
Halamková, Renata ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
1 ABSTRACT This bachelor theses deals with anaemia, one of the most common hematological diseases. The introductory part of this work defines the possible causes of this disease and its clinical picture. The following part includes the logical division of different types of anaemia accompanied with examples of real cases. Main focus of the theses was put on sideropenic and megaloblastic anaemia, as they occur considerably in the population.
|
|
|
Laboratory diagnostics of multiple myeloma.
Hubálková, Blanka ; Kovařík, Miroslav (advisor) ; Vrbacký, Filip (referee)
Multiple myeloma is one of the main representatives of monoclonal gammapathy diseases. It is caused by malignant transformation of B-lymphocytes, its clonal proliferation and accumulation of terminal stages of plasmocyte maturation. Clinical diagnosis is based on presence of this monoclonal immunoglobulin in serum or urine, myeloma cells infiltration into bone marrow and finding osteolytic lesions in bones. The electrophoresis and immunofixation of monoclonal immunoglobulins, free light chains assay, cytology of bone marrow aspirate are the most important laboratory tests helping in the statement of this immunoglbuline. The proof of the osteolytic damage is made using imaging technologies, such as RTG. Treatment of multiple myeloma had significantly improved during last years. The main options of therapy include high-dose chemotherapy and autological transplantations, together with immune modulating drugs, such as thalidomide, lenalidomide and bortezomib. Experimental part was focused on the comparison of sensitivity of paraprotein assessment by electrophoresis, immunofixation and free light chains assay. We can assume on the base of the results of the work that the free light chain assay is less sensitive method compared to k/I index. In addition - the higher sensitivity of IFO in comparison with...
|
guest ::
