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Effect of temperature and solution concentration on the properties of spray-dried lactose using the nozzle with diameter of 1,4 mm.
Vařilová, Tereza ; Svačinová, Petra (advisor) ; Vraníková, Barbora (referee)
2 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Consultant: PharmDr. Petra Svačinová, Ph.D. Student: Tereza Vařilová Title of Thesis: The influence of drying temperature and solution concentration on the properties of spray-dried lactose with the use of (using) nozzle with diameter 1,4 mm During spray drying the liquid is converted into solid particles. The properties of these particles can be modified by setting different drying parameters and properties of the used solution, suspension or emulsion. In this work, the influence of temperature and concentration of the solution on the properties of spray-dried lactose particles were measured using a nozzle with a diameter of 1.4 mm. The concentrations of the lactose solutions were 15% and 20%. The temperatures of spray drying were 210 řC and 170 řC for the solution with a concentration of 15 %, and 210 řC - 150 řC for the solution with a concentration of 20 %. Geometric properties of prepared particles were evaluated using an optical microscope. The thermal characteristics of the particles were evaluated using differential scanning calorimetry (DSC). The results showed that the particles are, unlike the initial material (D-lactose monohydrate), spherical and without surface irregularities. At both...
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Effect of temperature and solution concentration on the properties of spray-dried lactose using the nozzle with diameter of 1,4 mm.
Vařilová, Tereza ; Svačinová, Petra (advisor) ; Vraníková, Barbora (referee)
2 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Consultant: PharmDr. Petra Svačinová, Ph.D. Student: Tereza Vařilová Title of Thesis: The influence of drying temperature and solution concentration on the properties of spray-dried lactose with the use of (using) nozzle with diameter 1,4 mm During spray drying the liquid is converted into solid particles. The properties of these particles can be modified by setting different drying parameters and properties of the used solution, suspension or emulsion. In this work, the influence of temperature and concentration of the solution on the properties of spray-dried lactose particles were measured using a nozzle with a diameter of 1.4 mm. The concentrations of the lactose solutions were 15% and 20%. The temperatures of spray drying were 210 řC and 170 řC for the solution with a concentration of 15 %, and 210 řC - 150 řC for the solution with a concentration of 20 %. Geometric properties of prepared particles were evaluated using an optical microscope. The thermal characteristics of the particles were evaluated using differential scanning calorimetry (DSC). The results showed that the particles are, unlike the initial material (D-lactose monohydrate), spherical and without surface irregularities. At both...
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High-performance chromatographic methods for determination of proteins
Vařilová, Tereza ; Pacáková, Věra (advisor) ; Feltl, Ladislav (referee) ; Coufal, Pavel (referee) ; Kučerová, Zdenka (referee)
Conclusions This Thesis gives an overview of high-performance riquid chromatography in various separation modes for a characterization of selected proteins. various affinity stationary phases were prepared for analysis of pepsin, seminal plasma proteins and selected glycoproteins' choice of the affinity system suitable for a particular purpose is always a comprex probrem in which number of aspects must be considered. properties of the proteins to be separated and ligands used had to be taken into an account. The separation system should not influence the biorogicai activity ofproteins separated. From this point of view it is clear that for any each individuar system is necessary to find specific conditions for I) immobilizationof ligand, and II) separation conditions. Stationary phases with immob'ized 3,5-diiodo-L-tyrosine exhibited sufÍicient selectivity, proved to be useful for analysis ofporcine pepsin A and are appricable to practical sampres of human pepsin' Reversed-phase crs corumn is suitable for anarysis ofpepsin fragments obtained after a-chymotrypsin digestion. A study oť interaction of various forms of pepsin and pepsinogen are essentiar because of their high importance as crinicar diagnostic markers and as stomach enzyme. "lA IŤ Aťfinity chromatography with heparin immobilized to Toyopearl...
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