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Finding of new prognostic and diagnostic markers of pediatric acute leukemia by expression profiling data analysis
Vášková, Martina
We used publicly available expression profiling data of pediatric acute lymphoblastic leukemia (ALL) to identify genes correlating with genotypic subtypes or risk of the relapse. We searched for genes that can be investigated on the protein level by flow cytometry. We identified molecules CD44 and CD27 correlating with TEL/AML1pos ALL, molecules CD49f and CD99 correlating with BCR/ABLpos and CD135 molecule correlating with MLLpos ALL. The correlation of other molecules was not confirmed (IL10RB and CD97) or it was not possible to detect their expression on protein level (CD103, ICOSL and TNFR2). Based on the expression of CD44 and CD27 we identified counterparts of TEL/AML1pos and TEL/AML1neg leukemic cells in nonmalignant bone marrow. Although CD27 is known as a marker of mature memory B cells we showed that CD27 is also expressed by malignant and non-malignant B precursors. We demonstrate that CD27 and CD44 delineate stages of B precursor development. The developmental sequence starts from undetermined progenitors, expressing CD44. Upon B lineage commitment, cells gain CD27 and lose CD44. The CD27pos CD44neg (CD27 single positive, 27SP) cells express RAG-1 and TDT. These cells correspond to TEL/AML1pos ALL. The development follows to CD27/CD44 double positive (27/44DP) stage, 44SP stage and to...
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Finding of new prognostic and diagnostic markers of pediatric acute leukemia by expression profiling data analysis
Vášková, Martina
We used publicly available expression profiling data of pediatric acute lymphoblastic leukemia (ALL) to identify genes correlating with genotypic subtypes or risk of the relapse. We searched for genes that can be investigated on the protein level by flow cytometry. We identified molecules CD44 and CD27 correlating with TEL/AML1pos ALL, molecules CD49f and CD99 correlating with BCR/ABLpos and CD135 molecule correlating with MLLpos ALL. The correlation of other molecules was not confirmed (IL10RB and CD97) or it was not possible to detect their expression on protein level (CD103, ICOSL and TNFR2). Based on the expression of CD44 and CD27 we identified counterparts of TEL/AML1pos and TEL/AML1neg leukemic cells in nonmalignant bone marrow. Although CD27 is known as a marker of mature memory B cells we showed that CD27 is also expressed by malignant and non-malignant B precursors. We demonstrate that CD27 and CD44 delineate stages of B precursor development. The developmental sequence starts from undetermined progenitors, expressing CD44. Upon B lineage commitment, cells gain CD27 and lose CD44. The CD27pos CD44neg (CD27 single positive, 27SP) cells express RAG-1 and TDT. These cells correspond to TEL/AML1pos ALL. The development follows to CD27/CD44 double positive (27/44DP) stage, 44SP stage and to...
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