|
| |
|
| |
|
| |
|
|
Diversity of noncultivation and difficult cultivation representatives of family Psalteriomonadidae (Excavata: Heterolobosea)
Uhrová, Kristýna ; Čepička, Ivan (advisor) ; Škaloud, Pavel (referee)
Heterolobosea is a group of amoeboflagellates, amoebae, and flagellates belonging to the supergroup Excavata. Approximately 150 species of Heterolobosea have been described, from which less than 20 are obligately anaerobic. Most anaerobic species belong to the family Psalteriomonadidae. The aim of this study was to examine the diversity of potentially uncultivable anaerobic heteroloboseids. We obtained multiple new isolates, mainly from freshwater hypoxic sediments, isolated DNA from fresh cultures and determined their SSU rRNA gene sequences using Psalteriomonadidae-specific primers. In total, we have analyzed sequences of 123 new strains, which represented six already described species of Psalteriomonadidae as well as 16 potentially new ones. However, most of the new species died during the early stages of culture. Our results suggest that a great diversity of uncultivable psalteriomonadids exists in the nature. Key words: diversity, SSU rRNA, Heterolobosea, cultivation, Psalteriomonadidae
|
|
|
Metodika řešení šikany na vybraných SŠ
Uhrová, Kristýna
This thesis deals with bullying in secondary school level, more precisely at selected secondary schools and their approach to solving this pathological social phenomenon. The work is divided into theoretical and practical. The theoretical part describes the various stages and types of bullying, as well as partial steps in the solution to ensure the best possible protection of individuals against this problem. The methods used in the theoretical part are the study of professional literature sources, creation of extracts and their analysis, synthesis and comparison. The research is based on monitoring procedures for dealing with bullying at selected secondary schools. Its aim is to identify ways and methods of solving this problem. Investigations will be conducted at selected secondary schools of the South Moravian Region via questionnaire with teachers. Work provides information on ways to address bullying in middle level education. The recommendations for practice will be formulated ground the found results.
|
|
| |
guest ::
RSS feed.