|
| |
|
|
Athletics in elementary school
Trávníček, Martin ; Vindušková, Jitka (advisor) ; Feher, Jan (referee)
THE TITLE OF THE THESIS Athletics in elementary school THE TARGET OF THE THESIS The target of the thesis is a comparison of four-year-grade performance in the chosen athletics disciplines reached by boys from the second grade of two elementary schools. In one of them expanded sport education is taught. Next we want to draw a comparison of the relation between pupils' extracurricular sport activity and their performance in the chosen athletics disciplines. THE METOD The thesis is elaborated as a observational study. Within the research the method of collecting data - panel survey is applied. Data of these athletics disciplines are elaborated in the research: running at 60 m, long jump, shot - put and high jump. There is also used the inquiry method on the subject of extracurricular sport activities. CONCLUSION The gathered data concerning the chosen athletics disciplines of pupils from the elementary school with expanded sport education prove a higher growth performance. From the inquiry was found out that two thirds of respondents engage in extracurricular sport activities and their performance grew more continuously in the chosen athletics disciplines than the performance of those respondents who did not devote to these activities. KEY WORDS Athletics, performance, elementary school, extracurricular sport...
|
|
|
Determinants of fusogenicity of Syncytin-1, cellular glycoprotein of retroviral origin
Trávníček, Martin ; Trejbalová, Kateřina (advisor) ; Zábranská, Helena (referee)
Syncytin-1 is an endogenous retroviral envelope glycoprotein specifically expressed in human placenta, where the protein was adopted for its physiological function. After interaction with specific receptors, transmembrane proteins ASCT1 and ASCT2, Syncytin-1 initiates cell-cell fusion leading to formation of multinucleated syncytiotrophoblast, which is essential for feto-maternal nutrients exchange. In this diploma thesis a new cell-cell fusion quantification assay was implemented for characterisation of Syncytin-1 fusion determinants. The assay uses Syncytin-1 and ASCT2 expressed separately with fragments of luciferase in heterologous cell-culture system. The assay enables to specifically quantify cell-cell fusions based on activity of reconstituted luciferase reporter. This study discovered new facts about the role of intracytoplasmic tail of Syncytin-1 in the process of the cell- cell fusion. This specific part of protein contains a tandem motif sensitive to changes in amino acid sequence that led to loss of fusogenic potential of Syncytin-1. It was further confirmed, that the protein Suppressyn works as an inhibitor of cell-cell fusions initiated by Syncytin-1. Suppressyn however does not bind to receptors of Syncytin-1 and the mechanism of its inhibition remains unsolved. Finally, it was demonstrated...
|
|
|
Interaction of transmembrane proteins ASCT1 and ASCT2 with retroviral envelope glycoproteins
Trávníček, Martin ; Trejbalová, Kateřina (advisor) ; Mělková, Zora (referee)
Transmembrane proteins ASCT1 and ASCT2 are ubiquitous neutral amino acid transporters. Apart from their transporter function in metabolically active cells, they also serve as receptors for a wide group of retroviruses. All retroviruses recognizing the transmembrane receptor ASCT2/ASCT1 share a similar env gene, encoding the envelope glycoprotein. Syncytin-1 is the envelope glycoprotein, encoded by human endogenous retrovirus type W, produced in placental cytotrophoblasts of primates, including human. Interaction of receptor binding domain of Syncytin-1 and specific extracellular region of ASCT2 is responsible for fusion of neighbouring cells and formation of multinucleated syncytiotrophoblast. The importance of syncytiotrophoblast lies in higher efficiency of feto-maternal exchange of nutrients and simultaneously in modulation of immune response of mother towards fetus. Defect in syncytiotrophoblast differentiation often leads to complications during pregnancy and impairs the proper development of embryo. Characterization of protein domains responsible for the interaction between Syncytin-1 and its receptors is important to uncover genetic causes of these pathologies. Furthermore, understanding the interaction helps us to clarify the mechanism of cell entry and explains the molecular basis of host...
|
|
|
Athletics in elementary school
Trávníček, Martin ; Vindušková, Jitka (advisor) ; Feher, Jan (referee)
THE TITLE OF THE THESIS Athletics in elementary school THE TARGET OF THE THESIS The target of the thesis is a comparison of four-year-grade performance in the chosen athletics disciplines reached by boys from the second grade of two elementary schools. In one of them expanded sport education is taught. Next we want to draw a comparison of the relation between pupils' extracurricular sport activity and their performance in the chosen athletics disciplines. THE METOD The thesis is elaborated as a observational study. Within the research the method of collecting data - panel survey is applied. Data of these athletics disciplines are elaborated in the research: running at 60 m, long jump, shot - put and high jump. There is also used the inquiry method on the subject of extracurricular sport activities. CONCLUSION The gathered data concerning the chosen athletics disciplines of pupils from the elementary school with expanded sport education prove a higher growth performance. From the inquiry was found out that two thirds of respondents engage in extracurricular sport activities and their performance grew more continuously in the chosen athletics disciplines than the performance of those respondents who did not devote to these activities. KEY WORDS Athletics, performance, elementary school, extracurricular sport...
|
|
| |
guest ::
RSS feed.