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The thermodynamics of DNA-hairpin formation
Sgallová, Ráchel ; Římal, Václav (advisor) ; Profant, Václav (referee)
Serum response factor (SRF) is a transcription factor which binds to a highly conserved DNA sequence called the CArG box. According to the nucleotide sequence of CArG box it could form a hairpin structure or a cruciform. In this master thesis, the structure of the CArG box in a human gene c-fos was studied by nuclear magnetic resonance. 1 H spectra at temperatures 274{356 K, two-dimensional 1 H{1 H NOESY spectra, and two-dimensional 1 H{13 C HMBC spectra for DNA sequences with lengths of 12, 14 and 16 nucleoti- des were acquired. The thermodynamic parameters of formation of the secondary structure in the samples were determined from the measured temperature depen- dencies. The hairpin formation in the samples was con rmed based on the NOESY spectra and the lack of dependency of the melting temperature on concentration. The observed difference of the secondary structure from B-DNA could serve as a possible explanation of the high a nity of SRF to CArG box. 1
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Study of LaCuAl3 structure by means of nuclear magnetic and quadrupole resonance
Sgallová, Ráchel ; Chlan, Vojtěch (advisor) ; Englich, Jiří (referee)
In the presented bachelor thesis the structure of LaCuAl3 was studied on a series of metallic powder samples LaCuxAl4−x, x = 0,75; 0,9; 1 and 1,1 especially with respect to the local symetry of La atoms. 63 Cu, 65 Cu, 27 Al and 139 La nuclear magnetic resonance spectra were measured at room tempera- ture in external magnetic eld of 9,4 T. Some of the measured spectra were compared with simulated ones in order to access the number of nonequiva- lent atoms in the structure. Furthermore, the effect of Al/Cu stoichiometry on nuclear magnetic resonance spectra was observed and diccussed. 1
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The thermodynamics of DNA-hairpin formation
Sgallová, Ráchel ; Římal, Václav (advisor) ; Profant, Václav (referee)
Serum response factor (SRF) is a transcription factor which binds to a highly conserved DNA sequence called the CArG box. According to the nucleotide sequence of CArG box it could form a hairpin structure or a cruciform. In this master thesis, the structure of the CArG box in a human gene c-fos was studied by nuclear magnetic resonance. 1 H spectra at temperatures 274{356 K, two-dimensional 1 H{1 H NOESY spectra, and two-dimensional 1 H{13 C HMBC spectra for DNA sequences with lengths of 12, 14 and 16 nucleoti- des were acquired. The thermodynamic parameters of formation of the secondary structure in the samples were determined from the measured temperature depen- dencies. The hairpin formation in the samples was con rmed based on the NOESY spectra and the lack of dependency of the melting temperature on concentration. The observed difference of the secondary structure from B-DNA could serve as a possible explanation of the high a nity of SRF to CArG box. 1
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Study of LaCuAl3 structure by means of nuclear magnetic and quadrupole resonance
Sgallová, Ráchel ; Chlan, Vojtěch (advisor) ; Englich, Jiří (referee)
In the presented bachelor thesis the structure of LaCuAl3 was studied on a series of metallic powder samples LaCuxAl4−x, x = 0,75; 0,9; 1 and 1,1 especially with respect to the local symetry of La atoms. 63 Cu, 65 Cu, 27 Al and 139 La nuclear magnetic resonance spectra were measured at room tempera- ture in external magnetic eld of 9,4 T. Some of the measured spectra were compared with simulated ones in order to access the number of nonequiva- lent atoms in the structure. Furthermore, the effect of Al/Cu stoichiometry on nuclear magnetic resonance spectra was observed and diccussed. 1
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