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Inhibitors of proteolytic enzymes of Trematodes
Šteiger, Vladimír ; Kašný, Martin (advisor) ; Salát, Jiří (referee)
i Abstract Trematodes are important parasites possessing various localization in the bodies of invertebrate and vertebrate hosts, including human; therefore they are subject of long time intensive worldwide research. Trematodes developed various adaptations and strategies (some of them have also molecular background) enable them to survive in the host bodies. Trematodes produce large amount of different molecules, which are involved in various physiological processes. Inhibitors of proteolytic enzymes form a large group of biologically active compounds, e.g. they regulate the activity of peptidases or modulate host immune response. Many of these inhibitors are investigated as potential candidates in chemotherapeutic fight against trematodes. This thesis reviews the information concerning the natural inhibitors produced by trematodes and also synthetic inhibitors. Key words: Inhibitor, trematode, peptidase, serpin, cystatin i
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Antigenic and enzymatic properties of sand fly saliva
Hostomská, Jitka ; Volf, Petr (advisor) ; Salát, Jiří (referee) ; Bilej, Martin (referee)
i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...
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Inhibitors of proteolytic enzymes of Trematodes
Šteiger, Vladimír ; Kašný, Martin (advisor) ; Salát, Jiří (referee)
i Abstract Trematodes are important parasites possessing various localization in the bodies of invertebrate and vertebrate hosts, including human; therefore they are subject of long time intensive worldwide research. Trematodes developed various adaptations and strategies (some of them have also molecular background) enable them to survive in the host bodies. Trematodes produce large amount of different molecules, which are involved in various physiological processes. Inhibitors of proteolytic enzymes form a large group of biologically active compounds, e.g. they regulate the activity of peptidases or modulate host immune response. Many of these inhibitors are investigated as potential candidates in chemotherapeutic fight against trematodes. This thesis reviews the information concerning the natural inhibitors produced by trematodes and also synthetic inhibitors. Key words: Inhibitor, trematode, peptidase, serpin, cystatin i
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Antigenic and enzymatic properties of sand fly saliva
Hostomská, Jitka ; Volf, Petr (advisor) ; Salát, Jiří (referee) ; Bilej, Martin (referee)
i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...
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