|
|
Preparation of Microbial Metabolites from Waste Materials
Zichová, Miroslava ; Márová, Ivana (referee) ; Stloukal, Radek (referee) ; Rosenberg, Michal (advisor)
In this thesis the use of waste materials for the microbial production of important metabolites is reported. The first part is focused on the use of waste paper (a lignocellulosic material) as a non-traditional source for the production of bioethanol. The second part is focused on the immobilization of cellulolytic enzymes, which are used for the hydrolysis of lignocellulosic materials. First, the waste paper (cardboard) was pre-treated using a blender and a vibratory mill. The pre-treated cardboard was used for the production of ethanol by the method of simultaneous saccharification and fermentation. This method was optimized with free cells of Saccharomyces cerevisiae. Then strains suitable for the immobilization were selected. Strains of S. cerevisiae and Pichia kudriavzevii were immobilized by encapsulation into the polyvinyl alcohol carrier and tested again for the ethanol production by simultaneous saccharification and fermentation. In the second part of the work a carrier from waste polyethylene terephthalate bottles was prepared and used for the immobilization of the cellulolytic complex. The basic characteristics were determined, such as optimal pH and optimal temperature, storage, operational and thermal stability, enzyme kinetics and the mode of action of the enzyme. Compared to two other commercial carriers this carrier showed to be suitable for the immobilization of the cellulolytic complex.
|
|
|
High-speed Networks in Household
Rosenberg, Michal ; Mačák, Jaromír (referee) ; Škorpil, Vladislav (advisor)
The master thesis discusses about the relation between high-speed networks and intelligent system installation features and possibilities of this mutual interaction. Furthermore principles of KNX bus tunneling through IP networks are theoretically analyzed (KNXnet/IP). Practical use of implements KNXnet/IP shows real elements on the test panel. The control is realized by KNX@Home in the Ubuntu environment. Simulation of the real state tunneling KNX bus represent model in Opnet Modeler, which shows how the delays may change due to network load.
|
|
|
Objective Exercise of a New Transmission Equipment
Rosenberg, Michal ; Polívka, Michal (referee) ; Škorpil, Vladislav (advisor)
The aim of my project is designing of the example of the new transmission equipment PCM30U - 3UST for education of the subject Access and transport networks in form of the three new laboratory tasks. The tasks are focused on the configuration of a more universal familiarity with transmission facilities-PCM30U-3UST. Students learn to operate with the equipment so that they will be able to manage its interface and practical usage of its insertable cards. Further they gain theoretical knowledge of the E1, that they verify it through the analyzer PUMA 4300E. This work is also acquainted them with the theoretical principles of signal transduction through the framework of the E1 and contains a brief manual of the transmission equipment PCM30U-3UST and analyzer PUMA 4300. Laboratory tasks are processed including detailed workflow, which includes graphic illustration of linkage and developed model protocols.
|
|
|
Preparation of Microbial Metabolites from Waste Materials
Zichová, Miroslava ; Márová, Ivana (referee) ; Stloukal, Radek (referee) ; Rosenberg, Michal (advisor)
In this thesis the use of waste materials for the microbial production of important metabolites is reported. The first part is focused on the use of waste paper (a lignocellulosic material) as a non-traditional source for the production of bioethanol. The second part is focused on the immobilization of cellulolytic enzymes, which are used for the hydrolysis of lignocellulosic materials. First, the waste paper (cardboard) was pre-treated using a blender and a vibratory mill. The pre-treated cardboard was used for the production of ethanol by the method of simultaneous saccharification and fermentation. This method was optimized with free cells of Saccharomyces cerevisiae. Then strains suitable for the immobilization were selected. Strains of S. cerevisiae and Pichia kudriavzevii were immobilized by encapsulation into the polyvinyl alcohol carrier and tested again for the ethanol production by simultaneous saccharification and fermentation. In the second part of the work a carrier from waste polyethylene terephthalate bottles was prepared and used for the immobilization of the cellulolytic complex. The basic characteristics were determined, such as optimal pH and optimal temperature, storage, operational and thermal stability, enzyme kinetics and the mode of action of the enzyme. Compared to two other commercial carriers this carrier showed to be suitable for the immobilization of the cellulolytic complex.
|
|
|
Objective Exercise of a New Transmission Equipment
Rosenberg, Michal ; Polívka, Michal (referee) ; Škorpil, Vladislav (advisor)
The aim of my project is designing of the example of the new transmission equipment PCM30U - 3UST for education of the subject Access and transport networks in form of the three new laboratory tasks. The tasks are focused on the configuration of a more universal familiarity with transmission facilities-PCM30U-3UST. Students learn to operate with the equipment so that they will be able to manage its interface and practical usage of its insertable cards. Further they gain theoretical knowledge of the E1, that they verify it through the analyzer PUMA 4300E. This work is also acquainted them with the theoretical principles of signal transduction through the framework of the E1 and contains a brief manual of the transmission equipment PCM30U-3UST and analyzer PUMA 4300. Laboratory tasks are processed including detailed workflow, which includes graphic illustration of linkage and developed model protocols.
|
|
|
High-speed Networks in Household
Rosenberg, Michal ; Mačák, Jaromír (referee) ; Škorpil, Vladislav (advisor)
The master thesis discusses about the relation between high-speed networks and intelligent system installation features and possibilities of this mutual interaction. Furthermore principles of KNX bus tunneling through IP networks are theoretically analyzed (KNXnet/IP). Practical use of implements KNXnet/IP shows real elements on the test panel. The control is realized by KNX@Home in the Ubuntu environment. Simulation of the real state tunneling KNX bus represent model in Opnet Modeler, which shows how the delays may change due to network load.
|
guest ::
RSS feed.