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Risk Analyse of the Expertising in the Czech Republic
Složilová, Lenka ; Růžičková, Michaela (referee) ; Telec, Ivo (advisor)
This diploma thesis deals with risk mapping and investigation in the expert activity. To obtain further information, the questionnaire survey will be addressed to the experts from the Czech Republic as a supportive material of the diploma thesis. The part of the thesis will be focused on analysis and comparison of expert activity in neighbouring countries of the Czech Republic. The identified risks will evaluated based on the analysis of expert activity current state. The outcome of the thesis will be assessment of the situation in the Czech Republic and suggestions of measures leading to elimination of the risks as well as to reduction the occurrence probability or mitigating the impact of risks realization.
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Renessaince residences of the Trčka of Lípa and the work of Antonio Ericer
RŮŽIČKOVÁ, Michaela
The master's thesis is a continuation of the bachelor's thesis, which monographically dealt with the so-called Trčkův castle located in the area of the Premonstratensian monastery in Želiv. The theme of the thesis will focus on the group of residences of the Trčka family from Lípa in the Vysočina region with an emphasis on the Renaissance phase of the buildings (Ledeč nad Sázavou, Světlá nad Sázavou and Želiv). The thesis will deal with the cultural and historical context of their creation and the mutual formal similarity of the buildings. Particular attention will be paid to the realisation of the Italian-born builder Antonio Ericer, who worked for the Trčka family from Lípa and was also in contact with the Rožmberk family and the lords of Hradec. The author will try to find possible models for some significant forms of the buildings in question or typologically related comparisons in relevant areas.
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Reference gene selection for mRNA quantification in Haemonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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Reference gene selection for mRNA quantification in Heamonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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Opportunities of montan tourism in region of Příbram
RŮŽIČKOVÁ, Michaela
The bachelor thesis deals with the problematics and structure of mining tourism in Příbramsko with a focus on the Mining Museum Příbram. The aim of this work is to create the basis for the development of mining tourism in Příbram, based on the analysis and findings, which are offered to the management of the organization, including its founder. The findings lead to the systematization of work and offer a further step towards the new modern museum practice and an attractive and progressive approach to visitors.
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Reference gene selection for mRNA quantification in Heamonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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Collective and Group Art Activities in Kindergarten
RŮŽIČKOVÁ, Michaela
The topic of the bachelor thesis is group and collective art activities in kindergarten. The theoretical part deals with the characteristic of preschool age and the framework educational program for pre-school education. It also describes methods and forms of teaching of art activities. Next chapter focuses on children's creative expression and its development. At the end of this section are described some of the traditional and non-traditional artistic techniques and work habits during the drawing and writing. The practical part consists of description of specific group and collective activities performed with children in kindergartens.
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Reference gene selection for mRNA quantification in Haemonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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Effective study of musical piece
Růžičková, Michaela ; HAJÓSSYOVÁ, Magdaléna (advisor) ; BÁRTA, Martin (referee)
This bachelor´s thesis is targeted on finding the most effective way of working on a musical piece. It aims especially on the work of an opera singer, however, the author is operating with knowledge acquired by studying also two other classical specializations - piano and conducting. By combining of these three musical departments, this thesis tries to find the fastest and concurrently the most precise way of how to prepare a musical piece in the best possible quality. This thesis also deals with the issue of public performace, the question of stage fright and how to deal with it.
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