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CA IX protein expression in urogenital tumors, mainly in renal clear cell carcinoma and urotelial carcinoma of lower urinary tract
Hyršl, Lubomír ; Babjuk, Marek (advisor) ; Pacík, Dalibor (referee) ; Hora, Milan (referee)
Except testicular tumors and prostate carcinoma, there is lack of tumor markers in urogenital tumors. Carbonic anhydrase IX protein (CA IX) is a cell membrane protein, which can be ectopically expressed in various human tumors. It is often expressed as a response of tumor cells to hypoxia, which escalates invasivity of the tumor. In consequence, CA IX was proposed as a marker of poor prognosis. Its presence was proved by immunohistochemistry in renal cell carcinoma (RCC) and transitional cell carcinoma (TCC) of urinary tract. We study CA IX as a possible tumor marker. Presence of CA IX protein in tumor tissue was examined by imunohistochemistry, in serum and urine CA IX concentrations by immunoprecipitation of CA IX followed by Western blotting in combination with enhanced chemiluminiscence or ELISA. From our results we proved positive concentrations of soluble CA IX (s-CA IX) in 74,3% serum and in 63,3% urine samples in RCC group. In TCC group s-CA IX was positive in 69,6 % urine samples. Serum concentrations were all negative in this group. There was rapid decrease of s-CA IX concentration in serum after surgical removal of tumor with half-time 1- 2 days. s-CA IX is not detectable in blood and urine in healthy volunteers. In these days s-CA IX is not suitable marker of RCC and TCC. Nevertheless,...
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CA IX protein expression in urogenital tumors, mainly in renal clear cell carcinoma and urotelial carcinoma of lower urinary tract
Hyršl, Lubomír ; Babjuk, Marek (advisor) ; Pacík, Dalibor (referee) ; Hora, Milan (referee)
Except testicular tumors and prostate carcinoma, there is lack of tumor markers in urogenital tumors. Carbonic anhydrase IX protein (CA IX) is a cell membrane protein, which can be ectopically expressed in various human tumors. It is often expressed as a response of tumor cells to hypoxia, which escalates invasivity of the tumor. In consequence, CA IX was proposed as a marker of poor prognosis. Its presence was proved by immunohistochemistry in renal cell carcinoma (RCC) and transitional cell carcinoma (TCC) of urinary tract. We study CA IX as a possible tumor marker. Presence of CA IX protein in tumor tissue was examined by imunohistochemistry, in serum and urine CA IX concentrations by immunoprecipitation of CA IX followed by Western blotting in combination with enhanced chemiluminiscence or ELISA. From our results we proved positive concentrations of soluble CA IX (s-CA IX) in 74,3% serum and in 63,3% urine samples in RCC group. In TCC group s-CA IX was positive in 69,6 % urine samples. Serum concentrations were all negative in this group. There was rapid decrease of s-CA IX concentration in serum after surgical removal of tumor with half-time 1- 2 days. s-CA IX is not detectable in blood and urine in healthy volunteers. In these days s-CA IX is not suitable marker of RCC and TCC. Nevertheless,...
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Possibilities of preoperative determination of local extent of prostate cancer
Záleský, Miroslav ; Urban, Michael (advisor) ; Jarolím, Ladislav (referee) ; Novák, Jaroslav (referee) ; Pacík, Dalibor (referee)
Author analyses results of methods of preoperative local staging of prostate cancer (digital rectal examination -DRE, prostate biopsy, statistical predictive nomograms etc.) Further he analyses results of imaging methods (CT, MRI, ultrasound and all of his modification- Doppler imaging, harmonic imaging, 3D reconstruction contrast enhancement).
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