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Immersion into Spirit and Fire: Exegesis Mt 3:1-12
Perner, Jan ; Mrázek, Jiří (advisor) ; Pokorný, Petr (referee)
The purpose of the thesis is to try to suggest an understandable translation of the text of Matthew 4:1-12 focusing on the secondhalf of the eleventh verse which is the top of the whole part. It deals with the appearance of John the Baptist in the desert. The most important part of his speech is the description of coming Messiah. The character of Messiah described by words "that one, who..." brings some problems in understanding , especially tthe function of initation. The work tries to describe it analytically and find a solution. Except for exegesis of this text, there is the view of new testament on baptism in general, the baptism in Holy Spirit, and baptism by fire including the description of some ways and opinions. The thesis looks for resources in modern commentaries and biblical lexicons, and also it compares other passages from bible with this topic, and last but not least, there is given my own view of understanding the text. In the last part there is a summary of the whole topic and some application for the church of today. It is possible to use it for Sunday service or for pastoral care in personal spiritual growth. Powered by TCPDF (www.tcpdf.org)
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Nutritional requirements of ticks.
PERNER, Jan
Ticks acquire nutrients only by a parasitic nature of feeding on animals, including humans. During this process, a wide array of pathogens is transmitted. Ticks of the Ixodidae family receive exactly one blood meal in each active developmental. Knowing the trophic dependence of tick metabolism on the host blood meal components may enable discovering processes essential for the tick physiology and development. Exploiting a membrane system of tick feeding and whole blood fractionation, we have revealed that adult ticks need to acquire host haemoglobin-derived haem so that they can produce viable larvae, and reproduce. Haem is not further catabolised in ticks, and iron is thus acquired via independent route with the host serum transferrin as a source molecule. Using RNA-seq, we compared transcriptome compositions between guts of blood- and serum-fed ticks. We identified fifteen gut transcripts that change their levels with respect to the presence/absence of dietary red blood cells. Glutathione S-transferase, one of the identified encoded molecules, shows a clear haeminresponsive expression at both transcript and protein levels. Its apparent haem-binding properties suggest that this protein is directly involved in haem homeostasis maintenance within the tick gut. The ultimate goal of such research is to identify and verify targets that, when blocked, would render the acquisition and/or distribution system of haem in ticks nonfunctional. This would represent a novel way of anti-tick interventions in veterinary and human medicine.
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Phenotyping of a glutamate dehydrogenase a null mutant of \kur{Plasmodium falciparum}
PERNER, Jan
Glutamate dehydrogenase a (GDHa) has been suggested as a potential drug target against the malaria parasite Plasmodium falciparum. GDHa knockout cell line was generated and needed a phenotypic description by means of molecular biology and biochemistry. The knockout cell line was tested for higher oxidative stress sensitivity, levels of relevant proteins and gene transcripts were quantified. Furthermore, concentrations of two key molecules enabling redox homeostasis, glutathione and NADPH, were attempted to quantify. Finally, we attempted to disrupt a gene of another glutamate dehydrogenase, gdhb, which did not result in formation of viable parasites. In conclusion, GDHa is not a suitable drug target and GDHb needs to be further elucidated.
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Characterization of cystatin in the tick \kur{Ixodes ricinus}
PERNER, Jan
In numerous organisms, proteases and their proteinaceous inhibitors constitute a major defence mechanism. As vectors are in constant touch with pathogens their proteolytic activity is of crucial importance. In Ixodes ricinus (IR), the main vector of Lyme disease in Europe, the midgut cells process bloodmeal particles as well as provide a major barrier to a pathogen invasion. Tick salivary secretion plays a indispensable role in both bloodmeal completion and inadvertant pathogen transmission. This thesis describes 3 IRcystatins, cysteine protease inhibitors, using standard biochemical and molecular protocols. Moreover, this work provides evidence that cystatin 3 is likely to be involved in gut processes. This study also highlights recent knowledge on tick physiology during blood-feeding.
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