Original title: Phenotyping of a glutamate dehydrogenase a null mutant of \kur{Plasmodium falciparum}
Translated title: Phenotyping of a glutamate dehydrogenase a null mutant of \kur{Plasmodium falciparum}
Authors: PERNER, Jan
Document type: Master’s theses
Year: 2011
Language: cze
Abstract: Glutamate dehydrogenase a (GDHa) has been suggested as a potential drug target against the malaria parasite Plasmodium falciparum. GDHa knockout cell line was generated and needed a phenotypic description by means of molecular biology and biochemistry. The knockout cell line was tested for higher oxidative stress sensitivity, levels of relevant proteins and gene transcripts were quantified. Furthermore, concentrations of two key molecules enabling redox homeostasis, glutathione and NADPH, were attempted to quantify. Finally, we attempted to disrupt a gene of another glutamate dehydrogenase, gdhb, which did not result in formation of viable parasites. In conclusion, GDHa is not a suitable drug target and GDHb needs to be further elucidated.
Keywords: antioxidant defence; glutamate dehydrogenase; malaria; plasmodium falciparum
Citation: PERNER, Jan. Phenotyping of a glutamate dehydrogenase a null mutant of \kur{Plasmodium falciparum}. České Budějovice, 2011. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/14886

Permalink: http://www.nusl.cz/ntk/nusl-55088


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses