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Optimization of PEI based LbL capsules with pDNA
Ondrašáková, Petra ; Mladěnka, Přemysl (advisor) ; Hofman, Jakub (referee)
Ab rac Charle Uni er i Fac l of Pharmac in Hradec Kr lo Depar men of Pharmacolog and To icolog S den : Pe ra Ondra ko , MSc. S per i or: A oc. Prof. P em l Mlad nka, Pharm.D., Ph.D. Co- per i or: Dr. Haider Sami, Uni er i of Vienna, Di i ion of Clinical Pharmac and Diagno ic Ti le of rigoro he i : Op imi a ion of PEI ba e LbL cap le i h pDNA In hi he i , e foc ed on he de elopmen of pol e h lenimine (PEI) ba ed non- iral cap le , hich ill en er n cle of he cancer cell and lead o he e pre ion of he lacking pro ein, hich ca e di ea e. A fir , PEI ba ed la er-b -la er (LbL) pol mer cap le ere formed. PEI ha man ad an age - i ha pro onable amine , good abili and ran fec ion efficienc . When forming he cap le, I follo ed p on m diploma he i and con in ed o op imi e he mo i able combina ion of pol mer , ha ere la ered on he CaCO3core. The aim a o ob ain a biodegradable cap le and hen o incorpora e pla mid DNA (pDNA) herein. Fl ore cen l labelled PEI a ed a he la la er o i ali e par icle in fl ore cen micro cope. Then a canning elec ron micro cope (SEM) a ed o ob er e cap le in more de ail . UV-VIS and Dapi aining ere ed o ee he her here i pDNA bo nd in cap le . Ne ep ere i i cell e perimen on mo e mammar cancer cell - c o o ici and cell lar in ake. Vi ali a ion a pro ided i h fl ore cen micro cop . 2 pe of...
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