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Utilization of liquid chromatography-mass spectrometry for the analysis of psychoactive substances in unconventional matrices
Nemeškalová, Alžběta ; Sýkora, David (advisor) ; Vrkoslav, Vladimír (referee)
Utilization of liquid chromatography-mass spectrometry for the analysis of psychoactive substances in unconventional matrices Abstract The growing popularity of new products used as alternatives to the traditional illegal drugs represents a current challenge for toxicological laboratories, which are required to widen the range of analytical methods and aim at new analytes and matrices. This work focuses on two types of analytes, specifically, amphetamine-like stimulants and cannabinoids, and their analysis in a variety of complex matrices. An LC-MS/MS method suitable for the analysis of amphetamine-like stimulants in newborn meconium and breast milk was developed. The sample extraction was based on the principles of salting-out assisted liquid-liquid extraction, which can be considered a potential alternative to conventional extraction techniques. The analysis was performed using a reversed-phase chromatography coupled to tandem mass spectrometry in multiple reaction monitoring mode, and the method was validated for six analytes, namely amphetamine, methamphetamine, 3,4- methylendioxymethamphetamine, mephedrone, methylone and 3,4- methylenedioxypyrovalerone. Finally, the analysis of authentic meconium and breast milk samples confirmed the applicability of the method. An LC-UV-MS/MS method for the analysis...
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Nanoparticle encapsulation of bacterial antigen for vaccination purposes
Nemeškalová, Alžběta ; Jílek, Petr (advisor) ; Konečná, Klára (referee)
Author: Alžběta Nemeškalová Title: Nanoparticle encapsulation of bacterial antigen for vaccination use Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Shigellosis, an infectious diarrheal disease caused by Shigella spp., is currently a major health concern in developing countries and there is still no vaccine available. Non-living vaccines seem to be the safest option: our approach is therefore based on using Outer membrane vesicles (OMVs) as a subunit vaccine. However, mucosally administered OMVs are not capable of inducing as appropriate protection and a suitable adjuvant must be added to modify the immune response. Polymeric nanoparticles (NP) were studied in this work as mucosal adjuvats. The aim of this project was to prepare particles based on a foodborne protein (Protein P, under patent). Apart from simple proteic particles, two different ligands (Compound A, Compound B) were used to modify the particle surface, which resulted in four different formulations (P, P-A, P-B, P-A-B). We loaded these NPs with OMVs and described their properties, such as size, polydispersity and Z-potential. Their capability to encapsulate and carry the bacterial antigens was also determined. Furthermore, a method of dissolving proteic NP was developed in order to...
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Nanoparticle encapsulation of bacterial antigen for vaccination purposes
Nemeškalová, Alžběta ; Jílek, Petr (advisor) ; Konečná, Klára (referee)
Author: Alžběta Nemeškalová Title: Nanoparticle encapsulation of bacterial antigen for vaccination use Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Shigellosis, an infectious diarrheal disease caused by Shigella spp., is currently a major health concern in developing countries and there is still no vaccine available. Non-living vaccines seem to be the safest option: our approach is therefore based on using Outer membrane vesicles (OMVs) as a subunit vaccine. However, mucosally administered OMVs are not capable of inducing as appropriate protection and a suitable adjuvant must be added to modify the immune response. Polymeric nanoparticles (NP) were studied in this work as mucosal adjuvats. The aim of this project was to prepare particles based on a foodborne protein (Protein P, under patent). Apart from simple proteic particles, two different ligands (Compound A, Compound B) were used to modify the particle surface, which resulted in four different formulations (P, P-A, P-B, P-A-B). We loaded these NPs with OMVs and described their properties, such as size, polydispersity and Z-potential. Their capability to encapsulate and carry the bacterial antigens was also determined. Furthermore, a method of dissolving proteic NP was developed in order to...
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