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Modern extraction techniques in connection with HPLC for analysis of contaminants
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Ventura, Karel (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Kholová Aneta Supervisor prof. RNDr. Dalibor Šatínský, Ph.D. Advisor PharmDr. Ivona Lhotská, Ph.D. Title of Doctoral Thesis Modern extraction techniques in connection with HPLC for analysis of contaminants A compilation of six publications is presented in this dissertation thesis. The work focuses on applying and comparing extraction sorbents to analyze contaminating substances in food and food supplements. The main aim of this thesis was to present micro and nanofibers as suitable extraction sorbents for use in analytical chemistry. For this purpose, the fibers were compared with other, mostly commercial, extraction sorbents suitable for the type of matrix tested. All experiments were carried out in the on-line connection of the extraction sorbent through the selection valve with the analytical column in the chromatographic system. A crucial part was also the use of the extraction properties of nanofibers for the analysis of real-life samples. In the first part, the extraction efficiency was studied on liquid matrices, where no need for complex matrix purification was required. The main aim was sufficient retention and...
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Modern extraction techniques in connection with HPLC for analysis of contaminants
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Ventura, Karel (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Kholová Aneta Supervisor prof. RNDr. Dalibor Šatínský, Ph.D. Advisor PharmDr. Ivona Lhotská, Ph.D. Title of Doctoral Thesis Modern extraction techniques in connection with HPLC for analysis of contaminants A compilation of six publications is presented in this dissertation thesis. The work focuses on applying and comparing extraction sorbents to analyze contaminating substances in food and food supplements. The main aim of this thesis was to present micro and nanofibers as suitable extraction sorbents for use in analytical chemistry. For this purpose, the fibers were compared with other, mostly commercial, extraction sorbents suitable for the type of matrix tested. All experiments were carried out in the on-line connection of the extraction sorbent through the selection valve with the analytical column in the chromatographic system. A crucial part was also the use of the extraction properties of nanofibers for the analysis of real-life samples. In the first part, the extraction efficiency was studied on liquid matrices, where no need for complex matrix purification was required. The main aim was sufficient retention and...
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Application of modern analytical methods in clinical research
Vernerová, Andrea ; Kujovská Krčmová, Lenka (advisor) ; Doležal, Rafael (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Andrea Vernerová Supervisor Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Advisor MUDr. Ondřej Sobotka, Ph.D. Title of Doctoral Thesis Application of modern analytical methods in clinical research The dissertation thesis, composed as a compilation of five publications, is dealing with the development, optimization and validation of chromatographic methods and extraction procedures for the determination of early activation of the immune system biomarkers (neopterin, kynurenine, and tryptophan) in saliva and gingival crevicular fluid in oncological patients and patients with periodontal diseases. Furthermore, the potential of the use of Clark's sensor to determine the correct function of platelets by measuring mitochondrial respiration and its application in clinical research is under investigation. This work was based on cooperation with several clinical departments of the University Hospital Hradec Králové and Olomouc, and Oroboros Instruments (Innsbruck, Austria). The theoretical part of the thesis is focused on detail description of the individual analytes, their clinical significance, used biological matrices and their...
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Application of modern analytical methods in clinical research
Vernerová, Andrea ; Kujovská Krčmová, Lenka (advisor) ; Doležal, Rafael (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Andrea Vernerová Supervisor Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Advisor MUDr. Ondřej Sobotka, Ph.D. Title of Doctoral Thesis Application of modern analytical methods in clinical research The dissertation thesis, composed as a compilation of five publications, is dealing with the development, optimization and validation of chromatographic methods and extraction procedures for the determination of early activation of the immune system biomarkers (neopterin, kynurenine, and tryptophan) in saliva and gingival crevicular fluid in oncological patients and patients with periodontal diseases. Furthermore, the potential of the use of Clark's sensor to determine the correct function of platelets by measuring mitochondrial respiration and its application in clinical research is under investigation. This work was based on cooperation with several clinical departments of the University Hospital Hradec Králové and Olomouc, and Oroboros Instruments (Innsbruck, Austria). The theoretical part of the thesis is focused on detail description of the individual analytes, their clinical significance, used biological matrices and their...
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Risks Associated with the Dietary Supplements Use.
Zubrová, Julie ; Jahodář, Luděk (advisor) ; Hronek, Miloslav (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Social and Clinical Pharmacy Candidate: PharmDr. Julie Zubrová Supervisor: prof. RNDr. Luděk Jahodář, CSc. Consultant: PharmDr. Jitka Pokladníková, Ph.D. Title of Thesis: Risks Associated with the Dietary Supplements Use The worldwide increase of dietary supplements use, the way they are marketed and the limited information about safety of these products open the space to make new studies and thus to increase the safety of dietary supplements use in general public. In this dissertation, four individual topics related to the dietary supplements use were addressed: the safety of the use of additives in dietary supplements, allergy-like reactions to herbal products in children, the quality of information on the Internet relating to dietary supplements, the dietary supplements use by patients in the pre-operative period. The results of individual studies carried out in the framework of the dissertation showed potential risks associated with the dietary supplements use. Besides undesirable side effects of dietary supplements and interactions, potential side effects have been reported with some additives, especially if the patient is receiving the additive simultaneously in several dietary supplements or with food....
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Modern chromatographic techniques in food contamination analysis
Lhotská, Ivona ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate Mgr. Ivona Lhotská Supervisor doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis Modern chromatographic techniques in food contamination analysis A compilation of seven publications dealing with chromatographic methods for determination of food contamination (particularly mycotoxins and artificial food colorants) is presented in dissertation thesis. All methods were fully validated, complying with the requirements and applied for real samples analysis. The first part describes the methods for mycotoxins determination based on on-line solid phase extraction (SPE) utilizing column-switching system. Reversed phase columns packed with superficially porous particles were chosen for both extraction and separation step during optimization of on-line SPE-HPLC method for ochratoxin A and citrinin determination in beer. In following work, determination of patulin in juices and zearalenone in beer, more selective molecularly imprinted polymers (MIP) were used for on-line extraction. Due to challenging optimization of on-line MIP SPE, the methods were compared to off-line MIP extraction and on-line extraction using conventional C18 phase to confirm the selectivity. The employed commercial MIP...
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Using of modern separation methods for clinical practice
Honegrová, Barbora ; Solich, Petr (advisor) ; Malíř, František (referee) ; Štěrbová, Petra (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate Mgr. Barbora Honegrová Supervisor prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis Using of modern separation methods for clinical practice This work is focused on the development of methods for the determination of vitamin A and E in biological materials using liquid chromatography. The theoretical background describes bioanalytical approaches to the determination of analytes from complex matrices. Sample preparation before analysis, chromatographic separation approaches and the validation of bioanalytical methods are discussed. The structure and biological function of target analytes are characterized. The experimental part contains three sections, two of them focus on the development of new chromatographic methods for the determination of vitamin A and E in biological samples such as serum, lipoprotein layers and breast milk. The aim is to find the most suitable conditions for the chromatographic separation of target analytes that will be used for clinical trials in University Hospital in Hradec Králové. The third section is concerned with monitoring the stability of vitamins in breast milk as well as the influence of pasteurisation and storage on levels of the target vitamins....
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Risks Associated with the Dietary Supplements Use.
Zubrová, Julie ; Jahodář, Luděk (advisor) ; Hronek, Miloslav (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Social and Clinical Pharmacy Candidate: PharmDr. Julie Zubrová Supervisor: prof. RNDr. Luděk Jahodář, CSc. Consultant: PharmDr. Jitka Pokladníková, Ph.D. Title of Thesis: Risks Associated with the Dietary Supplements Use The worldwide increase of dietary supplements use, the way they are marketed and the limited information about safety of these products open the space to make new studies and thus to increase the safety of dietary supplements use in general public. In this dissertation, four individual topics related to the dietary supplements use were addressed: the safety of the use of additives in dietary supplements, allergy-like reactions to herbal products in children, the quality of information on the Internet relating to dietary supplements, the dietary supplements use by patients in the pre-operative period. The results of individual studies carried out in the framework of the dissertation showed potential risks associated with the dietary supplements use. Besides undesirable side effects of dietary supplements and interactions, potential side effects have been reported with some additives, especially if the patient is receiving the additive simultaneously in several dietary supplements or with food....
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Modern chromatographic techniques in food contamination analysis
Lhotská, Ivona ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate Mgr. Ivona Lhotská Supervisor doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis Modern chromatographic techniques in food contamination analysis A compilation of seven publications dealing with chromatographic methods for determination of food contamination (particularly mycotoxins and artificial food colorants) is presented in dissertation thesis. All methods were fully validated, complying with the requirements and applied for real samples analysis. The first part describes the methods for mycotoxins determination based on on-line solid phase extraction (SPE) utilizing column-switching system. Reversed phase columns packed with superficially porous particles were chosen for both extraction and separation step during optimization of on-line SPE-HPLC method for ochratoxin A and citrinin determination in beer. In following work, determination of patulin in juices and zearalenone in beer, more selective molecularly imprinted polymers (MIP) were used for on-line extraction. Due to challenging optimization of on-line MIP SPE, the methods were compared to off-line MIP extraction and on-line extraction using conventional C18 phase to confirm the selectivity. The employed commercial MIP...
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Modern chromatographic techniques in food contamination analysis
Lhotská, Ivona ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate Mgr. Ivona Lhotská Supervisor doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis Modern chromatographic techniques in food contamination analysis A compilation of seven publications dealing with chromatographic methods for determination of food contamination (particularly mycotoxins and artificial food colorants) is presented in dissertation thesis. All methods were fully validated, complying with the requirements and applied for real samples analysis. The first part describes the methods for mycotoxins determination based on on-line solid phase extraction (SPE) utilizing column-switching system. Reversed phase columns packed with superficially porous particles were chosen for both extraction and separation step during optimization of on-line SPE-HPLC method for ochratoxin A and citrinin determination in beer. In following work, determination of patulin in juices and zearalenone in beer, more selective molecularly imprinted polymers (MIP) were used for on-line extraction. Due to challenging optimization of on-line MIP SPE, the methods were compared to off-line MIP extraction and on-line extraction using conventional C18 phase to confirm the selectivity. The employed commercial MIP...
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