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The effect of resveratrol and gambogic acid on the DNA damage caused by daunorubicin in neonatal rat cardiomyocytes.
Mašín, Martin ; Jirkovská, Anna (advisor) ; Pávek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Martin Mašín Supervisor: PharmDr. Anna Jirkovská, Ph.D. Title of diploma thesis: The effect of resveratrol and gambogic acid on the DNA damage caused by daunorubicin in neonatal rat cardiomyocytes. DNA Topoisomerases comprise a family of enzymes that are able to alter DNA topology by transient single- or double-strand breaks (DSB) during fundamental processes such as replication and transcription. Inhibition of topoisomerase II (TOP II) is the main mechanism of action of some antitumour drugs, such as anthracyclines (ANT; e.g., daunorubicin). They stabilize the DNA-TOP II complex, leading to the formation of DSBs and later to apoptosis. Other inhibitors, that interact with the enzyme without the DSB formation, can modulate the effect of ANT. In this thesis, we studied the DNA damage caused by daunorubicin (DAU) and its main metabolite daunorubicinol (DAUnol) and the effect of two naturally-derived compounds and TOP II catalytic inhibitors resveratrol (RES) and gambogic acid (GA) in neonatal rat cardiomyocytes. The DNA damage was determined as the extent of histone H2AX phosphorylation (γ-H2AX) and by Comet Assay. It can be concluded that both DAU and DAUnol (1,2 μM) exhibit DNA damage that is...
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The effect of resveratrol and gambogic acid on the DNA damage caused by daunorubicin in neonatal rat cardiomyocytes.
Mašín, Martin ; Jirkovská, Anna (advisor) ; Pávek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Martin Mašín Supervisor: PharmDr. Anna Jirkovská, Ph.D. Title of diploma thesis: The effect of resveratrol and gambogic acid on the DNA damage caused by daunorubicin in neonatal rat cardiomyocytes. DNA Topoisomerases comprise a family of enzymes that are able to alter DNA topology by transient single- or double-strand breaks (DSB) during fundamental processes such as replication and transcription. Inhibition of topoisomerase II (TOP II) is the main mechanism of action of some antitumour drugs, such as anthracyclines (ANT; e.g., daunorubicin). They stabilize the DNA-TOP II complex, leading to the formation of DSBs and later to apoptosis. Other inhibitors, that interact with the enzyme without the DSB formation, can modulate the effect of ANT. In this thesis, we studied the DNA damage caused by daunorubicin (DAU) and its main metabolite daunorubicinol (DAUnol) and the effect of two naturally-derived compounds and TOP II catalytic inhibitors resveratrol (RES) and gambogic acid (GA) in neonatal rat cardiomyocytes. The DNA damage was determined as the extent of histone H2AX phosphorylation (γ-H2AX) and by Comet Assay. It can be concluded that both DAU and DAUnol (1,2 μM) exhibit DNA damage that is...
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The possibilities of influence sensoric characteristics of common carp flesh
MAŠÍN, Milan
Organoleptic characteristics of fish meat are one of criteria, that make higher quality prerequisites for successful selling, especially for more exacting customers. An objective of this work is evaluate temperature and storage time aspects on these characteristics. Like an appropriate fish was selected Carp (Cyprinus carpio), weighing around 1,5 {--} 3 kg. In own sauce roast meat samples were sensoric analysed directly after killing and than in different time periods. The storage temperature was 3; 5 and -18 °C. Panel of evaluators judged odour, taste, flavour and consistence of fish meat. A protocol with an unstructured graphic scale was used for expressing the intensity of perception. Accomplishments were graphic and statistic processed.
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Controlling in the managing of DHL company
Mašín, Marek ; Štůsek, Jaromír (advisor) ; Král, Jan (referee)
Bachelor thesis named Controlling in managing of DHL company is focused on controlling tool and it is focused on assessing of value and practical use of this mentioned tool to ensure an improvement of the company.
First part of bachelor thesis will acquaint readers about general theoretical base of controlling and then the following part of introduction gets reader closer to more specific area of this tool and that is financial controlling.
Second part of bachelor thesis is focused on practical application and use of financial controlling in the international company, especially acquaint readers about position of controlling department in the company's organization tree, the individual activities including liability and responsibilities comes from the activity concerned.
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