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Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method I
Márová, Marie ; Křivčíková, Lucie (advisor) ; Vacková, Jana (referee)
Author: Marie Márová Supervisor: Ing. Lucie Křivčíková Title of diploma thesis: Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method I Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study program: Pharmacy The aim of our thesis was to evaluate activity of potentially antibiotic substances on eight bacteria strains. We have tested 42 substances, all of them were synthesized at the Department of Inorganic And Organic Chemistry of Faculty of Pharmacy in Hradec Králové. We have used the microdilution broth method for the evaluation. It is a standardize procedure for finding the minimal inhibitive concentration (MIC) of antibiotics (ATB) and thereby the quantitative sensitivity of bacteria to ATB. The principle of the method is the visual readout and evaluation of bacterial growth in wells of microtiter plate. The wells were filled with a live broth, an antibiotic substance and a microbe. The antibiotic effect of the substance was evaluated positive if it came to the visible inhibition of microbial growth in the well. We have tested derivative of sulphonamides and salicylanilides. The substances were divided into 13 groups according to their structure. We have recorded the highest effect by derivatives N-phenyl-2-hydroxybenzamide,...
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Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method
Márová, Marie ; Vejsová, Marcela (advisor) ; Jílek, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Department of Biological and Medical Sciences Candidate: Mgr. Marie Márová Consultant: Mgr. Marcela Vejsová, Ph.D. Title of Thesis: Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method The aim of the present thesis was to test 35 substances and evaluate their antimicrobial activity. The substances were divided by their structure in 10 groups, it concerned carbamates and benzoates of salicylanilides, derivates of mercaptobenzothiazole and derivates of other structures. The substances were tested on 8 bacterial and 8 fungal strains. We used microdilution broth method for testing. This method serves to quantitative determination of sensitivity of the strain to the tested substance, minimal inhibitive concentration (MIC) indicates the sensitivity rate of microb. The holes of microtitration plate were filled with growth medium, tested substance in gradually decreasing concentrations and suspension of the strain. MIC conforms to the concentration of the substance which suppresses the growth of the colony and its hole stayed clear after the incubation. The strongest antibacterial effect was noticed by the substances of the 6th group (thiocarbamates of...
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Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method
Márová, Marie ; Vejsová, Marcela (advisor) ; Jílek, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Department of Biological and Medical Sciences Candidate: Mgr. Marie Márová Consultant: Mgr. Marcela Vejsová, Ph.D. Title of Thesis: Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method The aim of the present thesis was to test 35 substances and evaluate their antimicrobial activity. The substances were divided by their structure in 10 groups, it concerned carbamates and benzoates of salicylanilides, derivates of mercaptobenzothiazole and derivates of other structures. The substances were tested on 8 bacterial and 8 fungal strains. We used microdilution broth method for testing. This method serves to quantitative determination of sensitivity of the strain to the tested substance, minimal inhibitive concentration (MIC) indicates the sensitivity rate of microb. The holes of microtitration plate were filled with growth medium, tested substance in gradually decreasing concentrations and suspension of the strain. MIC conforms to the concentration of the substance which suppresses the growth of the colony and its hole stayed clear after the incubation. The strongest antibacterial effect was noticed by the substances of the 6th group (thiocarbamates of...
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Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method I
Márová, Marie ; Křivčíková, Lucie (advisor) ; Vacková, Jana (referee)
Author: Marie Márová Supervisor: Ing. Lucie Křivčíková Title of diploma thesis: Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method I Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study program: Pharmacy The aim of our thesis was to evaluate activity of potentially antibiotic substances on eight bacteria strains. We have tested 42 substances, all of them were synthesized at the Department of Inorganic And Organic Chemistry of Faculty of Pharmacy in Hradec Králové. We have used the microdilution broth method for the evaluation. It is a standardize procedure for finding the minimal inhibitive concentration (MIC) of antibiotics (ATB) and thereby the quantitative sensitivity of bacteria to ATB. The principle of the method is the visual readout and evaluation of bacterial growth in wells of microtiter plate. The wells were filled with a live broth, an antibiotic substance and a microbe. The antibiotic effect of the substance was evaluated positive if it came to the visible inhibition of microbial growth in the well. We have tested derivative of sulphonamides and salicylanilides. The substances were divided into 13 groups according to their structure. We have recorded the highest effect by derivatives N-phenyl-2-hydroxybenzamide,...
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