Home > Search Results: Kratochvílová, Michala
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
miRNA analysis of small extracellular vesicles isolated from plasma after diet intervention
 Kratochvílová, Michala ; Koc, Michal (advisor) ; Šrámek, Jan (referee)
The aim of this thesis was to establish a protocol for the isolation of small extracellular vesicles (sEVs) from human plasma. Plasma samples were collected from 44 healthy women who underwent a 60-hour dietary intervention in the form of fasting as part of a clinical trial. These women were divided into two groups based on their BMI - lean and obese. Plasma was collected from each participant before and after the dietary intervention and sEVs were isolated using the polyethylene glycol precipitation method. Each sample was then characterized by the size of sEVs and their concentration. The amount of sEVs in plasma before the dietary intervention was the same for both groups and did not change with dietary intervention. However, the 60-hour fasting resulted in a change in sEVs size in the lean group. Another goal of this thesis was to analyze miRNA expression in sEVs. There were differences found in the expression of certain miRNA between the two groups. More specifically, differences were observed in the expression of hsa-miR-17-5p, hsa-miR-20a-5p and hsa-let-7b-5p. The 60-hour fasting period resulted in an increased expression of hsa-miR-5100 in the lean group and hsa- miR-27a-3p and hsa-miR-27b-3p in the obese group. However, these changes induced by the dietary intervention were not evaluated...
Detailed record

See also: similar author names
16 KRATOCHVÍLOVÁ, Marie
9 KRATOCHVÍLOVÁ, Markéta
20 KRATOCHVÍLOVÁ, Martina
5 KRATOCHVÍLOVÁ, Michaela
2 KRATOCHVÍLOVÁ, Miroslava
17 KRATOCHVÍLOVÁ, Monika
9 Kratochvilová, Markéta
17 Kratochvilová, Monika
3 Kratochvílová, Magdalena
6 Kratochvílová, Marcela
16 Kratochvílová, Marie
9 Kratochvílová, Markéta
2 Kratochvílová, Marta
20 Kratochvílová, Martina
5 Kratochvílová, Michaela
17 Kratochvílová, Monika
Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English