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On-line marketing communication
Kollár, Jan ; Chlebovský, Vít (referee) ; Schüller, David (advisor)
Obsah této bakalářské práce se zabývá marketingovou komunikací obchodů EXE Jeans. Tyto obchody prodávají smíšené zboží od více značek a jsou rozšířeny v oblasti celé České republiky. V první části práce jsou pokryty a vysvětleny teoretické pojmy úzce spjaty se zadaným tématem. V druhé, praktické části se pak dále zaměřuji na analýzu současného stavu vybrané společnosti, jejího prostředí a možných zlepšení v návrhové části, které jsou hlavním cílem této práce.
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Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers
Kollár, Jan ; Zimčík, Petr (advisor) ; Mosinger, Jiří (referee) ; Zitko, Jan (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Author: Mgr. Jan Kollar Supervisor: prof. PharmDr. Petr Zimcik, Ph.D. Advisor: doc. PharmDr. Veronika Novakova, Ph.D. Doctoral Thesis: Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers Phthalocyanines (Pcs) and their aza-analogues (e.g. tetrapyrazinoporphyrazines, TPyzPzs) represent a promising group of organic dyes with interesting photophysical properties (strong absorption in area 650-750 nm, strong singlet oxygen production) highly suitable for the use in photodynamic therapy (PDT) of cancer. However, they have also some drawbacks lowering their potential use in PDT (low solubility in water, strong tendency to aggregate). The topic of my dissertation thesis closely follows the topic of my diploma thesis, during which TPyzPz with sixteen carboxylate groups in rigid arrangement was prepared and in which we have disclosed strong negative effect of low pH and serum proteins on photodynamic activity. In the first part of this thesis I prepared new zinc TPyzPz with eight sulfonate groups on periphery, which was characterised by good solubility in water. Sulfonates were chosen as stronger acids than carboxylic groups, so they were...
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Synthesis of azaphthalocyanines substituted with moieties derived from trimesic acid
Kollár, Jan ; Zimčík, Petr (advisor) ; Roh, Jaroslav (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Author: Jan Kollár Supervisor: Doc. PharmDr. Petr Zimčík, PhD. Title of diploma thesis: Synthesis of azaphthalocyanines substituted with moieties derived from trimesic acid Aim of this work was a synthesis of new photosensitizers from the group of water- soluble azaphthalocyanines (AzaPc) with future potential to be used in PDT. The starting compound for synthesis, trimesic acid, was esterified by ethanol and the triester was partially hydrolysed to mono carboxylic acid. Subsequently, the carboxyl group was selectively reduced to hydroxyl followed by oxidation to aldehyde. Benzoin condensation of this aldehyde gave acyloin that was oxidized to tetraethyl 5,5'-oxalyldiisophthalate. Substituted pyrazine-2,3-dicarbonitrile, a precursor for AzaPc, was obtained by condensation of diaminomaleonitirile with this vicinal diketon. Cyclotetramerization using magnesium butoxide as initiator gave magnesium(II) AzaPc substituted with sixteen butoxycarbonyl groups. Magnesium complexes were converted to metal-free AzaPc in acidic medium and then to zinc(II) AzaPc by reaction with anhydrous zinc acetate. Tetraethyl 5,5'-oxalyldiisophthalate was also hydrolyzed to diketon with four free...
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Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers
Holmanová, Pavlína ; Macháček, Miloslav (advisor) ; Kollár, Jan (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Author: Bc. Pavlína Holmanová Supervisor: RNDr. Miloslav Macháček, Ph.D. Consultant: RNDr. Klára Konečná, Ph.D. Title of diploma thesis: Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers Background: This work is aimed to optimize methodological approaches for the evaluation of the antimicrobial activity of selected representative (aza)phthalocyanine photosensitizers (PSs) in vitro. In addition, the in vitro antimicrobial activity of candidate PSs was evaluated against three clinically important pathogens. Namely, gram positive methicillin resistant bacterium Staphylococcus aureus (MRSA), gram negative bacterium Pseudomonas aeruginosa (PA), and yeast Candida albicans (CA) were included in the study. Methods: Microorganisms were incubated with the selected PSs, which were subsequently washed away, and microorganisms with bound PSs were irradiated for 15 or 30 min. At the same time, the intrinsic antimicrobial activities of the studied compounds without irradiation were determined as well. Three different methodological approaches were used to evaluate the minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC). The Bioscreen C instrument was used for...
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Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers
Holmanová, Pavlína ; Macháček, Miloslav (advisor) ; Kollár, Jan (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Author: Bc. Pavlína Holmanová Supervisor: RNDr. Miloslav Macháček, Ph.D. Consultant: RNDr. Klára Konečná, Ph.D. Title of diploma thesis: Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers Background: This work is aimed to optimize methodological approaches for the evaluation of the antimicrobial activity of selected representative (aza)phthalocyanine photosensitizers (PSs) in vitro. In addition, the in vitro antimicrobial activity of candidate PSs was evaluated against three clinically important pathogens. Namely, gram positive methicillin resistant bacterium Staphylococcus aureus (MRSA), gram negative bacterium Pseudomonas aeruginosa (PA), and yeast Candida albicans (CA) were included in the study. Methods: Microorganisms were incubated with the selected PSs, which were subsequently washed away, and microorganisms with bound PSs were irradiated for 15 or 30 min. At the same time, the intrinsic antimicrobial activities of the studied compounds without irradiation were determined as well. Three different methodological approaches were used to evaluate the minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC). The Bioscreen C instrument was used for...
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On-line marketing communication
Kollár, Jan ; Chlebovský, Vít (referee) ; Schüller, David (advisor)
Obsah této bakalářské práce se zabývá marketingovou komunikací obchodů EXE Jeans. Tyto obchody prodávají smíšené zboží od více značek a jsou rozšířeny v oblasti celé České republiky. V první části práce jsou pokryty a vysvětleny teoretické pojmy úzce spjaty se zadaným tématem. V druhé, praktické části se pak dále zaměřuji na analýzu současného stavu vybrané společnosti, jejího prostředí a možných zlepšení v návrhové části, které jsou hlavním cílem této práce.
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Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers
Kollár, Jan ; Zimčík, Petr (advisor) ; Mosinger, Jiří (referee) ; Zitko, Jan (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Author: Mgr. Jan Kollar Supervisor: prof. PharmDr. Petr Zimcik, Ph.D. Advisor: doc. PharmDr. Veronika Novakova, Ph.D. Doctoral Thesis: Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers Phthalocyanines (Pcs) and their aza-analogues (e.g. tetrapyrazinoporphyrazines, TPyzPzs) represent a promising group of organic dyes with interesting photophysical properties (strong absorption in area 650-750 nm, strong singlet oxygen production) highly suitable for the use in photodynamic therapy (PDT) of cancer. However, they have also some drawbacks lowering their potential use in PDT (low solubility in water, strong tendency to aggregate). The topic of my dissertation thesis closely follows the topic of my diploma thesis, during which TPyzPz with sixteen carboxylate groups in rigid arrangement was prepared and in which we have disclosed strong negative effect of low pH and serum proteins on photodynamic activity. In the first part of this thesis I prepared new zinc TPyzPz with eight sulfonate groups on periphery, which was characterised by good solubility in water. Sulfonates were chosen as stronger acids than carboxylic groups, so they were...
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Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers
Kollár, Jan ; Zimčík, Petr (advisor) ; Mosinger, Jiří (referee) ; Zitko, Jan (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Author: Mgr. Jan Kollar Supervisor: prof. PharmDr. Petr Zimcik, Ph.D. Advisor: doc. PharmDr. Veronika Novakova, Ph.D. Doctoral Thesis: Synthesis of anionic phthalocyanines as potential photodynamic active photosensitizers Phthalocyanines (Pcs) and their aza-analogues (e.g. tetrapyrazinoporphyrazines, TPyzPzs) represent a promising group of organic dyes with interesting photophysical properties (strong absorption in area 650-750 nm, strong singlet oxygen production) highly suitable for the use in photodynamic therapy (PDT) of cancer. However, they have also some drawbacks lowering their potential use in PDT (low solubility in water, strong tendency to aggregate). The topic of my dissertation thesis closely follows the topic of my diploma thesis, during which TPyzPz with sixteen carboxylate groups in rigid arrangement was prepared and in which we have disclosed strong negative effect of low pH and serum proteins on photodynamic activity. In the first part of this thesis I prepared new zinc TPyzPz with eight sulfonate groups on periphery, which was characterised by good solubility in water. Sulfonates were chosen as stronger acids than carboxylic groups, so they were...
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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) I have obtained 3 lineages in molecular marker trnGucc group II intron. I did not acquire significant and homogenous results in SSU intron. It is probable that the lineages of trnGucc intron are very young. SEM revealed one central pore in cell wall and five bulges. The lineages were significantly different in their shape and dimensions. The lineages differentiated mainly in their length and breadth of the cells. The lineages inside morphotype E. humerosum differentiated in shape and dimensions of the polar lobe. The lineages inside morphotype E.didelta differentiated in shape and dimensions of the neck. That was ascertained using the geometric morphometrics with the measuring. Linear discriminant analysis of the desmids from literature revealed that is possible to partly discriminate groups of the varieties (E. humerosum var. parallelum and E. didelta in lineage A; two formae E. didelta f. val Piora and E. didelta f. latior in lineage B; E. didelta var. inermiforme and E. humerosum var. affine in lineage C). Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) we have obtained 3 lineages in molecular marker trnGuuc group II intron. We did not acquire significant and homogenous results in SSU intron. It is probable that the lineages are very young. The lineages were significantly different in their shape and dimensions. We used the geometric morphometrics with the measuring of the lengths and breath of the semicells, isthmus and their ratios. The lineages differentiated in their dimensions and shape variable polar lobes and neck. SEM revealed the one central pore in cell wall on the contrary, with variant E. didelta var. bengalicum, which has two pores according the literature records. Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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