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The usage of the rubber dam in restorative dentistry and endodontics
Kapitán, Martin ; Koberová Ivančaková, Romana (advisor) ; Roubalíková, Lenka (referee) ; Netolický, Jan (referee)
The usage of the rubber dam in restorative dentistry and endodontics - abstract MUDr. Martin Kapitán Good isolation of the working field is a condition for high quality, safe, efficient and successful work in restorative dentistry and endodontics. The use of rubber dam is considered as a standard of care in the isolation of working field. Rubber dam is a rubber membrane with holes around the crowns of isolated teeth. It separates the working field from the other parts of the oral cavity. Rubber dam protects the patients from injuries and aspiration or ingestion of small foreign bodies. It enables suction of all cooling water, preparation debris and all agents used during the treatment. It steadily provides clear access and overview of the operation field. It protects the working field from saliva contamination. It plays an important role in infection control. In spite of repetitive recommendations of dentistry authorities the use of rubber dam is not widespread, even though the most frequent arguments of dental practitioners against rubber dam have been disproved in many studies. The aims of this work were (i) to find out the frequency and circumstances of rubber dam use among dentists in the Czech Republic and to compare the data with other countries, (ii) to reveal the patients' attitudes to rubber dam...
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Účinek antimikrobiálních prostředků na mikroorganismy dutiny ústní
Kudiyirickal, Marina George ; Koberová Ivančaková, Romana (advisor) ; Hamal, Petr (referee) ; Poleník, Pavel (referee)
Disease of the pulp and periodontium which constitute the vast proportion of odontogenic infections are mainly caused by the endogenous bacterial microbiota in the oral cavity while non odontogenic infections in the same area vary depending on the nature and site of infection. The rational use of antibiotic is important to prevent development of resistant strains and other side effects of drugs. Aim: To investigate (i) the prevalence of bacterial species in oral samples of patients with bacterial infection reporting at the Dept of Dentistry (1996- 2007), (ii) to assess the age and sex predilection and , (iii) and species specific relationships, and (iv) to determine the susceptible-resistant biotype profile of the bacterial isolates from odontogenic and non odontogenic infections. Materials and methods: Laboratory and clinical data of patient's electronic files at Dept of Microbiology, Faculty of Medicine and Teaching Hospital in Hradec Králové for the years 1996-2007 were evaluated retrospectively. Results: Bacterial orofacial odontogenic or non odontogenic infection was detected in a total of 678 patients with 350 males (51.6%) and 328 females (48.4%). The bacterial isolates included 48 bacterial species with predominance of facultative anaerobes which accounted for 78.5% (n= 1263) and obligate anaerobes...
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Dental pulp stem cells
Suchánek, Jakub ; Koberová Ivančaková, Romana (advisor) ; Kukletová, Martina (referee) ; Hampl, Aleš (referee)
The aim of this dissertation study was to optimize the isolation and long term cultivation protocols for human dental pulp stem cells. The protocols which showed best results were used for cultivation of dental pulp stem cell isolated from exfoliated teeth (SHED). Additional aims were to characterize DPSC and SHED and prove their ability to proliferate over Hayflick's limit and differentiate into mature cell lines (osteoblasts, chondroblasts and adipocytes). In order to find optimal protocols for isolation of dental pulp from tooth, we tried three different approaches. During optimization of cultivation protocol we focused on decreasing amount of fetal calf serum (FCS) from 10 % FCS in cultivation media (most often used in literature) into 2 % and thus get closer to cultivation conditions suitable for clinical usage. We compared DPSC cultivated in three different media (medium with 10 % FCS, 2 % FCS supplemented with growth factors and media with 2 % FCS supplemented with ITS and growth factors). For characterization of DPSC and SHED we used basic biological properties (proliferation activity, viability, morphology), their phenotype and karyotype. The study demonstrated that the best protocol for isolation of dental pulp from tooth was to break the roots and extract the pulp throw this aperture. We...
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