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Modern extraction techniques in connection with HPLC for analysis of contaminants
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Ventura, Karel (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Kholová Aneta Supervisor prof. RNDr. Dalibor Šatínský, Ph.D. Advisor PharmDr. Ivona Lhotská, Ph.D. Title of Doctoral Thesis Modern extraction techniques in connection with HPLC for analysis of contaminants A compilation of six publications is presented in this dissertation thesis. The work focuses on applying and comparing extraction sorbents to analyze contaminating substances in food and food supplements. The main aim of this thesis was to present micro and nanofibers as suitable extraction sorbents for use in analytical chemistry. For this purpose, the fibers were compared with other, mostly commercial, extraction sorbents suitable for the type of matrix tested. All experiments were carried out in the on-line connection of the extraction sorbent through the selection valve with the analytical column in the chromatographic system. A crucial part was also the use of the extraction properties of nanofibers for the analysis of real-life samples. In the first part, the extraction efficiency was studied on liquid matrices, where no need for complex matrix purification was required. The main aim was sufficient retention and...
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Modern extraction techniques in connection with HPLC for analysis of contaminants
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Ventura, Karel (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Kholová Aneta Supervisor prof. RNDr. Dalibor Šatínský, Ph.D. Advisor PharmDr. Ivona Lhotská, Ph.D. Title of Doctoral Thesis Modern extraction techniques in connection with HPLC for analysis of contaminants A compilation of six publications is presented in this dissertation thesis. The work focuses on applying and comparing extraction sorbents to analyze contaminating substances in food and food supplements. The main aim of this thesis was to present micro and nanofibers as suitable extraction sorbents for use in analytical chemistry. For this purpose, the fibers were compared with other, mostly commercial, extraction sorbents suitable for the type of matrix tested. All experiments were carried out in the on-line connection of the extraction sorbent through the selection valve with the analytical column in the chromatographic system. A crucial part was also the use of the extraction properties of nanofibers for the analysis of real-life samples. In the first part, the extraction efficiency was studied on liquid matrices, where no need for complex matrix purification was required. The main aim was sufficient retention and...
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Determination of mycotoxin citrin using on-line SPE HPLC on molecularly imprinted polymers
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Aneta Kholová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: Determination of mycotoxin citrinin using on-line SPE HPLC on molecularly imprinted polymers The aim of this work was to develop a fast method for mycotoxin citrinin (CIT) determination using high performance liquid chromatography (HPLC) and column switching technique in combination with on-line solid phase extraction (SPE) based on citrinin-selective molecularly imprinted polymer (MIP). The most suitable sorbent for selective extraction was chosen from six newly synthetized MIP after evaluation of their binding capacity and selectivity. Selectivity was tested by comparing molecularly imprinted and non-imprinted polymers and the ability of the MIP polymer to selectively separate citrinin from interferences of the test sample. The best MIP (functional monomer of metacrylamide with porogen acetonitrile) was filled into pre-column (20 × 3 mm) and connected to HPLC column-switching system. Three different matrices were injected to chromatographic system. 50 μl of the sample was injected onto MIP extraction column and washed out of interferences with methanol / 0.5% water solution of acetic acid in a ratio of...
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Determination of mycotoxin citrin using on-line SPE HPLC on molecularly imprinted polymers
Kholová, Aneta ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Aneta Kholová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: Determination of mycotoxin citrinin using on-line SPE HPLC on molecularly imprinted polymers The aim of this work was to develop a fast method for mycotoxin citrinin (CIT) determination using high performance liquid chromatography (HPLC) and column switching technique in combination with on-line solid phase extraction (SPE) based on citrinin-selective molecularly imprinted polymer (MIP). The most suitable sorbent for selective extraction was chosen from six newly synthetized MIP after evaluation of their binding capacity and selectivity. Selectivity was tested by comparing molecularly imprinted and non-imprinted polymers and the ability of the MIP polymer to selectively separate citrinin from interferences of the test sample. The best MIP (functional monomer of metacrylamide with porogen acetonitrile) was filled into pre-column (20 × 3 mm) and connected to HPLC column-switching system. Three different matrices were injected to chromatographic system. 50 μl of the sample was injected onto MIP extraction column and washed out of interferences with methanol / 0.5% water solution of acetic acid in a ratio of...
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