|
|
P-selectin expression in brachiocephalic artery during atherogenesis
Kadlecová, Magda ; Nachtigal, Petr (advisor) ; Blažíčková, Kateřina (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences P-selectin expression in brachiocephalic artery during atherogenesis Diploma thesis Magda Kadlecová Supervisor: doc. PharmDr. Petr Nachtigal, Ph.D. Background: We studied the expression of P-selectin in brachiocephalic artery in the apoE−/− /LDLr−/− deficient mice, which were fed with the low carbohydrate, high protein diet. Methods: For this study we used the female apoE−/− /LDLr−/− deficient mice, which were divided into two groups, 6 mice in each group. Control mice were fed with AIN-93G diet (control group) and the second group was fed by low carbohydrate, high protein diet for 8 weeks. Immunohistochemical analysis was performed in tissue sections of brachiocephalic artery by avidin-biotin complex method with DAB detection. Results: Biochemical analysis revealed significantly increased levels of total cholesterol after feeding with low carbohydrate, high protein diet. Immunohistochemical analysis detected P- selectin expression only in endothelial cells, with more intensity in endothelial cells covering atherosclerotic plaques in both groups apoE−/− /LDLr−/− deficient mice. Atherosclerotic plaques were found in all mice in LCHP group. In control group, there were few mice without...
|
|
|
P-selectin expression in brachiocephalic artery during atherogenesis
Kadlecová, Magda ; Nachtigal, Petr (advisor) ; Blažíčková, Kateřina (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences P-selectin expression in brachiocephalic artery during atherogenesis Diploma thesis Magda Kadlecová Supervisor: doc. PharmDr. Petr Nachtigal, Ph.D. Background: We studied the expression of P-selectin in brachiocephalic artery in the apoE−/− /LDLr−/− deficient mice, which were fed with the low carbohydrate, high protein diet. Methods: For this study we used the female apoE−/− /LDLr−/− deficient mice, which were divided into two groups, 6 mice in each group. Control mice were fed with AIN-93G diet (control group) and the second group was fed by low carbohydrate, high protein diet for 8 weeks. Immunohistochemical analysis was performed in tissue sections of brachiocephalic artery by avidin-biotin complex method with DAB detection. Results: Biochemical analysis revealed significantly increased levels of total cholesterol after feeding with low carbohydrate, high protein diet. Immunohistochemical analysis detected P- selectin expression only in endothelial cells, with more intensity in endothelial cells covering atherosclerotic plaques in both groups apoE−/− /LDLr−/− deficient mice. Atherosclerotic plaques were found in all mice in LCHP group. In control group, there were few mice without...
|
guest ::
RSS feed.