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Ecotoxicity of cholesterol-lowering-drugs
Königsmarková, Kristýna ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis is focused on the ecotoxicological testing of blood cholesterol lowering drugs. At first it considers the issue from a theoretical point of view – describes the effect of residual drugs on the environment, the distribution of hypolipidemics, the risks of elevated cholesterol, then deals with ecotoxicology and introduces the principles of ecotoxicological testing. In the experimental part, these following drugs were tested: Corsim, Atoris, Lipanthyl Supra, Lipanthyl 267M. In addition, clofibric acid – the metabolite of fibrates was tested. Residues of these drugs are found mainly in the aquatic ecosystem, that is why tests of aquatic organisms have been used to evaluate ecotoxicity. Test organisms were Thamnocephalus platyurus, Lemna minor and Vibrio fischeri. LC50, EC50 and IC50 values were determined on the basis of test results. Using these values, the ecotoxicity of the above mentioned hypolipidemics was evaluated.
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Microfluidic Enzymatic Reactor for Drug Screening
Königsmarková, Kristýna ; Peš,, Ondřej (referee) ; Přikryl,, Jan (advisor)
This master thesis deals with the use of microfluidics for the purpose of microfluidic enzymatic reactor for drug screening. At first it considers the issue from a theoretical point of view – describes microfluidics as a newly developing and promising field of production of microfluidic devices, materials, biomedical applications and advantages and disadvantages of microfluidics overall. Furthermore, it focuses on an area of analytical utilization of enzymes within enzyme reactors. In the first part of the experimental section, conditions for the testing of enzymes of xenobiotics metabolism in the liver were optimized, namely the model of coumarin metabolism via the spectrofluorimetry method. The second part of the experimental work dealt with optimization of the fabrication conditions of microfluidic chips from OSTE (off-stoichiometry Thiol Ene) via the soft lithography method. Subsequently, the functionality of the produced chips was tested. Based on the results of both parts of the experimental work, an evaluation was carried out to assess the suitability of their interconnection for future research – screening of microsomal enzyme activity and model biotransformation of drugs within the channels of the fabricated devices.
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Microfluidic Enzymatic Reactor for Drug Screening
Königsmarková, Kristýna ; Peš,, Ondřej (referee) ; Přikryl,, Jan (advisor)
This master thesis deals with the use of microfluidics for the purpose of microfluidic enzymatic reactor for drug screening. At first it considers the issue from a theoretical point of view – describes microfluidics as a newly developing and promising field of production of microfluidic devices, materials, biomedical applications and advantages and disadvantages of microfluidics overall. Furthermore, it focuses on an area of analytical utilization of enzymes within enzyme reactors. In the first part of the experimental section, conditions for the testing of enzymes of xenobiotics metabolism in the liver were optimized, namely the model of coumarin metabolism via the spectrofluorimetry method. The second part of the experimental work dealt with optimization of the fabrication conditions of microfluidic chips from OSTE (off-stoichiometry Thiol Ene) via the soft lithography method. Subsequently, the functionality of the produced chips was tested. Based on the results of both parts of the experimental work, an evaluation was carried out to assess the suitability of their interconnection for future research – screening of microsomal enzyme activity and model biotransformation of drugs within the channels of the fabricated devices.
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Ecotoxicity of cholesterol-lowering-drugs
Königsmarková, Kristýna ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis is focused on the ecotoxicological testing of blood cholesterol lowering drugs. At first it considers the issue from a theoretical point of view – describes the effect of residual drugs on the environment, the distribution of hypolipidemics, the risks of elevated cholesterol, then deals with ecotoxicology and introduces the principles of ecotoxicological testing. In the experimental part, these following drugs were tested: Corsim, Atoris, Lipanthyl Supra, Lipanthyl 267M. In addition, clofibric acid – the metabolite of fibrates was tested. Residues of these drugs are found mainly in the aquatic ecosystem, that is why tests of aquatic organisms have been used to evaluate ecotoxicity. Test organisms were Thamnocephalus platyurus, Lemna minor and Vibrio fischeri. LC50, EC50 and IC50 values were determined on the basis of test results. Using these values, the ecotoxicity of the above mentioned hypolipidemics was evaluated.
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