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A study of separation conditions and stability of selected phenolic compounds
Jandorová, Zuzana ; Nováková, Lucie (advisor) ; Kočová Vlčková, Hana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zuzana Jandorová Supervisor: Prof. PharmDr. Lucie Nováková, Ph.D. Title of the diploma thesis: A study of separation conditions and stability of selected phenolic compounds The goal of this diploma thesis was to determine the most suitable separation conditions for the analysis of ten phenolic compounds by the ultra-high performance liquid chromatography with PDA detection. The thesis is focused mainly on the separation of critical pair tamarixetin and isorhamnetin. Another goal was to determine the short-term stability of all tested compounds for 24 hours. Analyses were performed using the ACQUITY UPLC chromatographic system with PDA detector at a wavelength of 275 nm. The first step in the study of separation conditions was the screening of 17 columns from several different manufacturers. Tested columns included ACE Excel C18, BEH C18, CSH C18, Arion Plus C18, Ascentis Express C18, Atlantis Premier BEH C18 AX, BEH Shield RP18, Cortecs Shield RP18, Ascentis Express RP-Amide, BEH Phenyl, CSH Phenyl-Hexyl, Kinetex Biphenyl, ACE Excel C18-AR, ACE Excel C18-PFP, CSH Fluoro-Phenyl, Ascentis Express F5, and Kinetex PFP. During the screening, 3 organic modifiers (acetonitrile, methanol and acetonitrile...
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