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Detection of soluble endogline in Lab-in-Syringe
Ilićová, Marie ; Svobodová, Zuzana (advisor) ; Chocholouš, Petr (referee)
The main target of the thesis was to develop an automated method for the isolation of endoglin from a sample using a magnetic immunosorbent in Lab-In-Syringe with subsequent immunodetection by affiblot. The theoretical part deals with endoglin, in its membrane and soluble form, especially with their connection to endothelial dysfunction. The principles of anti-endoglin therapy using the monoclonal antibody carotuximab or TRC105 are also briefly described here. Furthermore, the theoretical part summarizes the basics of the automation in Lab-In-Syringe and of the affiblot technique, their principles, and their applications. The last chapter deals with the biofunctionalization of magnetic particles by antibodies in order to prepare a magnetic immunosorbent which is used for the isolation of proteins from a complex sample. The experimental part describes methods for testing the affiblot prototypes. The main goal was to develop a method for the preparation of anti-endoglin magnetic immunosorbent and to increase the capacity of the method by upscaling it from a 1 ml to a 5 ml syringe. Furthermore, methods for the isolation of the soluble form of human endoglin from the culture medium containing the drug TRC105 were developed for 1 ml and 5 ml syringes. Various experiments were carried out to optimize the...
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Alzheimer's disease - progress in therapy and diagnostics
Ilićová, Marie ; Svobodová, Zuzana (advisor) ; Herink, Josef (referee)
The bachelor thesis compiled the up-to-date theoretical knowledge about Alzheimer's disease focused on its diagnosis and treatment. The first part of the thesis is devoted to the dementia syndrome and Alzheimer's disease in general. The clinical signs, stages and forms of Alzheimer's disease are described here, and there is also briefly summarized information about prevalence and risk factors. The next part discusses the pathophysiology and pathogenesis of Alzheimer's disease, and the main hypotheses of the disease development are also described in this part. The chapter dealing with diagnostics is divided into diagnostic process, diagnostic criteria, laboratory tests and imaging methods. The chapter mainly describes the already established clinical, laboratory and imaging methods used in the clinical practice as well as methods that could be introduced into clinical practice in the future. Scientific research focuses on biomarkers related to pathological process of Alzheimer's disease. In the last part of bachelor's thesis are presented the possibilities of the therapy, including the symptomatic treatment, even the advances in treatment of amyloid β deposition. There are also mentioned considered directions of therapy in the research phase. Key words: dementia, amyloid plaques, neurofibrillary...
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