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Vzájemné ovlivňování angličtiny a druhého cizího jazyka a jejich vliv na jazykovou úroveň studentů na českých středních školách
Humhalová, Tereza ; Mikuláš, Martin (advisor) ; Dvořák, Bohuslav (referee)
My diploma thesis is concerned with the possible effects of linguistic transfer between a Czech grammar school students' acquired languages, namely English and French or German, on their performance in these languages. The thesis is written on the backdrop of the Czech Education System. The System of Curricular Documents and the related documents which predetermine the students' language development are introduced, and their most important implications for the development of the students' L2 and L3 are listed out. The foreign languages which this thesis is predominantly focused on, namely English, French and German, are analysed diachronically, synchronically and typologically to establish their main principles, their similarities, and differences, which may serve as the possible sources of linguistic transfer. Cognitive processes underlying the development of L2 and L3 systems in the context of the Czech Education System and relevant theories are introduced, including the variables which determine to a significant degree the development of these languages and may have implications for the possible instances of linguistic transfer. Hypotheses and research questions which arise from the theoretical research are then proposed, the answers to which I intend to provide by means of a...
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Membraneless organelles - physiological functions and experimental techniques of their study
Bašta, Miroslav ; Bařinka, Cyril (advisor) ; Humhalová, Tereza (referee)
Membraneless organelles, also called biomolecular condensates or protein droplets, are liquid spheric bodies present in an every cell compartment. Their composition and density is only slightly different from their surroundings. They consist of hundreds of types of proteins and nucleic acids and they are responsible for various biological functions. They are formed via liquid-liquid phase separation that creates a phase boundary in a solution of macromolecules in order to decrease the low free energy of the system. This process is initiated by external stress, internal cell processes or mutations in DNA. There are many identified types of membraneless organelles and each year there are more added on the list. Their functions include localization of macromolecules and related biochemical reactions, tuning of biochemical reactions and transport of macromolecules throughout the cell. This thesis presents a brief summary of the topic of membraneless organelles with several specific examples and very briefly describes several selected methods of their study.
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Characterization of WASH complex member protein SWIP
Humhalová, Tereza ; Libusová, Lenka (advisor) ; Lánský, Zdeněk (referee)
WASH complex regulates actin dynamics on endosomes by activating the Arp2/3 complex, which subsequently induces generation of branched actin patches. WASH complex is required for proper recycling of many important transmembrane proteins. Although the general physiological function of WASH complex is known, the role of its single subunits have not yet been adequately specified. This work focuses on one of these subunits - protein SWIP. This protein maintains vesicular localization of some WASH complex subunits in the slime mold Dictyostelium discoideum and a point mutation in its sequence causes a severe neurodegenerative disease - autosomal recessive intellectual disorder (ARID). Our results show that SWIP truncation results in its inability to incorporate into WASH complex. However, the C-terminal part of SWIP is able to localize onto intracellular vesicles, which are not WASH complex positive. We have also studied the impact of ARID-causing SWIP mutation, and we show, that it does neither change the protein's ability to bind the complex nor the overall localization of WASH complex.
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