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Design and synthesis of surface architectures on fluorescent nanodiamonds
Havlík, Jan
anks to their unique properties and high biocompatibilities, fluorescent nanodiamonds are promising representatives of modern carbon nanomaterials with a broad range of applications. Nevertheless, their wider use is limited because of weak fluorescence intensity and low colloidal stability in the biological environment. e optimization of treatment procedures and development of new suitable surface designs is therefore critically needed. In this study, several key steps for fluorescent nanodiamond treatment have been optimized, leading to both a substantial increase in fluorescence intensity and to significantly lower surface damage caused by graphitization. Further, a new high-throughput irradiation technique was developed. e influence of surface chemistry on the fluorescence parameters was studied using partial fluorination of the functional groups on the nanodiamond surface. A novel method which significantly affects the interaction of nanodiamonds with biological systems by increasing of the homogeneity and circularity was developed. e potential of nanodiamonds for future medical and biological research was demonstrated on particles with complex surface architectures that enabled targeting and therapy of tumor cells. Moreover, a strong and highly selective affinity of fibroblast growth factors to diamond...
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Contrast agents for hereronuclear MRI
Koucký, Filip ; Kotek, Jan (advisor) ; Havlík, Jan (referee)
In this thesis the pH dependency of the coordination modes of lanthanide complexes with macrocyclic ligands based on 1,4,7,10-tetraazacyclododecane skeleton was studied. The cyclen-based ligand structures in this work contained three acetate and one aminoethyl group with a N-alkyl-N-methylphosphonate substituent, DO3AN(R)P, where R is an alkyle substituent on the nitrogen atom of the pendant arm (R = methyl, benzyl). Lanthanide complexes of a previously studied prototype ligand DO3ANP with secondary amino group (R = H) have shown interesting properties in the field of 31 P NMR imaging because of their various coordination properties, which allow in situ pH measurement. These complexes can also be used for monitoring the kinetics of the chemical exchange of the amino group proton in 1 H NMR imaging using the chemical exchange saturation transfer (CEST NMR). In this thesis, two new derivatives DO3ANMeP and DO3ANBnP were prepared in order to better understand the coordination modes changes in this ligand series. Also, their coordination behaviour with selected lanthanide ions was studied (Eu3+ , Gd3+ , Dy3+ , Yb3+ ). Based on a series of NMR and luminescence measurements, it was found out, that in acidic conditions the complexes containing DO3ANP motif bind a water molecule in their coordination...
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Design and synthesis of surface architectures on fluorescent nanodiamonds
Havlík, Jan ; Kotek, Jan (advisor) ; Lang, Kamil (referee) ; Štěpánek, František (referee)
anks to their unique properties and high biocompatibilities, fluorescent nanodiamonds are promising representatives of modern carbon nanomaterials with a broad range of applications. Nevertheless, their wider use is limited because of weak fluorescence intensity and low colloidal stability in the biological environment. e optimization of treatment procedures and development of new suitable surface designs is therefore critically needed. In this study, several key steps for fluorescent nanodiamond treatment have been optimized, leading to both a substantial increase in fluorescence intensity and to significantly lower surface damage caused by graphitization. Further, a new high-throughput irradiation technique was developed. e influence of surface chemistry on the fluorescence parameters was studied using partial fluorination of the functional groups on the nanodiamond surface. A novel method which significantly affects the interaction of nanodiamonds with biological systems by increasing of the homogeneity and circularity was developed. e potential of nanodiamonds for future medical and biological research was demonstrated on particles with complex surface architectures that enabled targeting and therapy of tumor cells. Moreover, a strong and highly selective affinity of fibroblast growth factors to diamond...
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Video Quality Monitoring Using NetFlow
Havlík, Jan ; Grégr, Matěj (referee) ; Matoušek, Petr (advisor)
This bachelor's thesis is about creating new tools for a video transfer quality monitoring. It consists of a client-server architecture, where the client is gathering video quality statistics and passes those statistics to the server. The server updates relevant NetFlow IPFIX records with these statistics. The project includes video encoding, packet encapsulation and Internet protocols related to this topic. The architecture is written in a C language for a UNIX operating system.
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The design and synthesis of selective copper(II) chelators
Havlík, Jan ; Kotek, Jan (advisor) ; Holzhauser, Petr (referee)
Title: Design and synthesis of selective copper(II) chelators Author: Jan Havlík Department: Department of Inorganic Chemistry, Faculty of Science Supervisor: assoc. prof. RNDr. Jan Kotek, Ph.D. Supervisor's e-mail address: modrej@natur.cuni.cz The aim of this work was to prepare selective copper(II) chelators. Within the frame of this thesis were synthesized 13 compounds, from which 7 are new. All compounds were fully characterized by NMR spectroscopy and mass spectrometry. Copper(II) complex of synthesized ligand 1,11-bis(methylphosphinic acid)cyclam, (1,11-dipin), was studied in thermodynamic and formation/decomplexation studies. For 1,11-dipin-copper(II) complex were determined values of rate constants and activation parameters. Keywords: selective chelator, copper, cyclam derivatives, macrocyclic ligands, methylphosphonate, methylphosphinate
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Použití sociálních média pro propagaci vzdělávacích služeb
Kolomatskyi, Artem ; Pavlíček, Antonín (advisor) ; Havlík, Jan (referee)
The aim of the thesis is to analyze the influence of the promotion in the social media for educational services. Current work covers all the definitions and overviews of the literature that is relevant for the understanding of the chosen topic. Theoretical data was followed by the examples of implementation of tools that could help the enterprises to promote the goods and services on the local market. The practical part of the thesis is standing on the research of the business that provides educational services in Ukraine and how investments in the development of social media tools are showing the rise of the sales for the company. I consider my work to be helpful in understanding all crucial areas in social media marketing. The aim of this study is to offer valuable insights into the development campaign process that would help Meat Studies to attract more students to educational services of the company.
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