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Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications
Kročilová, Nikola ; Bačáková, Lucie ; Pařízek, Martin ; Havlíková, Jana ; Motarjemi, H. ; Molitor, M. ; Gabor, R. ; Marvan, J.
Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells.
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Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
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Marketing plan and it’s creation in the company CTY Průmyslová, s.r.o.
Larkina, Svetlana ; Procházková, Markéta (advisor) ; Gabor, Richard (referee)
The subject of the Master's Thesis is retrieval information needed to making a marketing plan in the company CTY Průmyslová, s.r.o. For this purpose the current situation at office real estate market in Prague was analyzed, used marketing tools were ascertained and was suggested the communication strategy for layout Průmyslová Tower to get new customers. Chapters in first part define a theoretical approach for creating a marketing plan. The second part focuses on a particular company because of preparation of the new building project and its subsequent introduction at the market.
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Project Management and Problem Solving Methods in Management Consulting
Gábor, Richard ; Hrůzová, Helena (advisor) ; Hayward, Stephen (referee)
Conducting management consulting project for Transparency International Czech Republic, the leading non-profit non-governmental organization active in anti-corruption practices, by applying selected project management and problem solving methods, the objective of the thesis is to support the assumption that proper selection and application of suitable methods to problem identification, definition and decomposition enables to come up with the solution of the problem by analyzing it with no need for further solution development.
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Selected problems in management consulting
Gábor, Richard ; Filipová, Alena (advisor) ; Zeman, Jiří (referee)
Nowadays, management consulting represents one of the primes of economy of knowledge. The beginning dates back to beginning of the classical management way, today's form shapes in early of this century. The factors, that have influenced today's form of this industry, are shakeout, commoditization and innovation. The first part of the thesis analyses these trends. The second part analyses the processes inside the management consulting. Those processes can be divided into those that lead to the completion of the contract and those that follow the completion of the contract. The thesis analyses the most frequently used methods, selects the most important analytical models and successful practices that help consultant in realization of a change that is the essence of the management consulting.
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