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Basics of current antimycobacterial treatment
Šilhavá, Kristýna ; Janďourek, Ondřej (advisor) ; Diepoltová, Adéla (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Pharmacy Author: Kristýna Šilhavá Supervisor: PharmDr, Ondřej Janďourek, Ph.D. Title of diploma thesis: Basics of current antimycobacterial therapy The aim of the theoretical part of this thesis is to summarize information regarding mycobacteria, mycobacterial infections and particularly their treatment. Infections caused by mycobacteria are widespread worldwide and a large part of the population is infected with them each year. The most common forms are pulmonary, but practically any organ of the human body can be affected. Although these diseases are treatable with combinations of antimycobacterial drugs, the number of cases with drug resistant forms of mycobacterial diseases is currently on the rise. Therefore, emphasis is placed on the development and testing of new drugs that would be able to treat these resistant forms of diseases in the future. The experimental part is dedicated to testing newly synthesized antimycobacterial compounds that were tested using the microdilution broth method against five strains of Mycobacterium genus (M. smegmatis, M. aurum, M. avium, M. kansasii and M. tuberculosis H37Ra). The results are interpreted and evaluated in terms of the...
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Optimization of the method for sensitivity evaluation of biofilm-forming staphylococci against candidate antimicrobial compounds
Diepoltová, Adéla ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Study program: Healthcare Bioanalytics Author: Bc. Adéla Diepoltová Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Optimization of the method for sensitivity evaluation of biofilm- forming staphylococci against candidate antimicrobial compounds Background: The aim of this thesis was to optimize approach for in vitro formation of staphylococcal biofilms on the pegs and on the wells of the 96-well panel as an analogous approach to commercially available Calgary Biofilm Device system. The aim of the Experiment 1 was to evaluate incubation conditions (such as impact of a growth medium, incubation mode, optical density of the starting bacterial inoculum and type of surface) leading to maximal biofilm formation of two biofilm producer strains with unknown biofilm phenotype and one staphylococcal strain known as strong biofilm producer. The most advisable conditions were used in incubation process of Experiment 2. This work should propose the approach leading to in vitro formation of the most voluminous staphylococcal biofilms exploitable for candidate drug antimicrobial activity testing. Methods: Spectrophotometric measurement of the crystal violet colour extracted from wells with fixed and stained Staphylococci to evaluate the ability to...
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Optimization of the method for sensitivity evaluation of biofilm-forming staphylococci against candidate antimicrobial compounds
Diepoltová, Adéla ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Study program: Healthcare Bioanalytics Author: Bc. Adéla Diepoltová Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Optimization of the method for sensitivity evaluation of biofilm- forming staphylococci against candidate antimicrobial compounds Background: The aim of this thesis was to optimize approach for in vitro formation of staphylococcal biofilms on the pegs and on the wells of the 96-well panel as an analogous approach to commercially available Calgary Biofilm Device system. The aim of the Experiment 1 was to evaluate incubation conditions (such as impact of a growth medium, incubation mode, optical density of the starting bacterial inoculum and type of surface) leading to maximal biofilm formation of two biofilm producer strains with unknown biofilm phenotype and one staphylococcal strain known as strong biofilm producer. The most advisable conditions were used in incubation process of Experiment 2. This work should propose the approach leading to in vitro formation of the most voluminous staphylococcal biofilms exploitable for candidate drug antimicrobial activity testing. Methods: Spectrophotometric measurement of the crystal violet colour extracted from wells with fixed and stained Staphylococci to evaluate the ability to...
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Pathogenesis of infectious diseases caused by biofilm forming microorganisms
Diepoltová, Adéla ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles Univerzity Faculty of Pharmacy in Hradec Králové Study program: Medical Laboratory Technician Author: Adéla Diepoltová Supervisor: RNDr. Klára Konečná, Ph.D. Title of bachelor thesis: Pathogenesis of infectious diseases caused by biofilm-forming microorganisms Background: The aim of this thesis is to describe the importance of microbial biofilms in medicine and in pathogenesis of infectious diseases. This thesis includes specification of the concept of biofilm, its structure and chemical composition, formation, development and processes occurring in biofilm which are directly related to pathogenesis of infectious diseases. Briefly, the most common infectious diseases associated with the formation of microbial biofilms are also mentioned. Main findings: Microbial biofilms are active layers of microorganisms and their metabolic products. Biofilm, unlike the planktonic phase, is sessile form of life. The microorganism first adheres to suitable substrate, then multiplies and produces metabolites, that create slime called matrix. Parts of the biofilm layer can tear off and colonize new environments. These environments include any natural niches with water access, industrial pipelines but host tissues, as well. Biofilms cause diseases such as otitis media, endocarditis, or pneumonia in patients...
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