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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Ellipsometry and fluorescence microscopy applied to model membranes
 Miszta, Adam ; Hof, Martin (advisor) ; Hudeček, Jiří (referee) ; Dejneka, Alexander (referee)
7 Abstract The following PhD thesis presents two optical experimental techniques: ellipsometry and confocal fluorescence microscopy, and focuses on their applications in investigation of supported phospholipid bilayers (SPBs). The thesis presents two studies. First one shows real-time effects of ethanol on supported phospholipid bilayers formed by adsorption of phospholipid vesicles on solid surface measured by ellipsometry. This study first time demonstrated that ethanol-induced membrane interdigitation, as demonstrated for free standing bilayers, does not occur in the supported membranes. The finding indicates that membranes formed on supports, even fused into confluent bilayers, may differ from free-standing bilayer in vesicles. The second study is based on the combination of ellipsometry with two fluorescence microscopy techniques (i.e.Laser Scanning Microscopy and Z-Scan Fluorescence Correlation Microscopy) to gain information on the interaction of antimicrobial peptides with SPBs. These techniques were used to compare the mechanisms of interaction of the two different antimicrobial peptides α-helical Magainin 2 and β-sheet Cryptdin-4. LSM shows appearance of inhomogeneities in spatial distribution of lipids in the bilayer after treatment with Crp-4. Ellipsometric measurements show that binding of...
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Z-scan Fluorescence Correlation Spectroscopy Applied in Studies of Model Phospholipid Membranes
 Kulakowska, Anna ; Hof, Martin (advisor) ; Konopásek, Ivo (referee) ; Dejneka, Alexander (referee)
6. Summary First of all, the issue whether and to what extent lipid mobility in the bilayer is altered when the bilayer is being deposited on the solid support was addressed. We designed a method that allows a direct comparison of the diffusion coefficients of labelled-lipids in the free-standing lipid bilayer of GUVs and that of the bilayer interacting with the mica surface (SPBs) in the glucose solution. The lateral diffusion analysis strongly indicate that the observed phenomenon is an unrestricted 2D diffusion. The results clearly show that the diffusion is slowed by more than 2 times for the interaction with the support. Moreover, we believe that the quantitative comparison of lipid diffusion in two frequently used model membranes can be helpful when comparing data reported in the literature. Additionally, the obtained data clearly indicate the existence of an inter-leaflet coupling. The lateral diffusion of investigated membrane probes and phospholipid analogues depend on the character and structure of the used fluorescent probe. The obtained lateral diffusion of the flavone probe, F2N12S is significantly faster than that of the fluorescent lipid analogues under study. Moreover, flavone probe exhibits approximately an order of magnitude faster flip-flop in comparison to the Atto633-DOPE lipid analogue...
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2 Dejneka, Alexandr
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