|
|
Cocultivation of yeasts and microalgae to produce enriched biomass
Bradáčová, Lenka ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The diploma thesis is focused on the influence of biological stress formed by co-cultivation of heterotrophic (yeasts) and autotrophic (microalgae and cyanobacteria) organisms on the production of enriched biomass. The monitored groups of substances include carotenoids (-carotene, lutein, lycopene, astaxanthin, torularhodin), chlorophylls A and B, ergosterol and ubiquinone. Further, production of lipids was analyzed in the terms of fatty acid profile and lipid content in biomass. In the first part of the work, the yeast biomass production was investigated using different nitrogen sources. Glycerol was used as a carbon source in all parts of the work. Subsequently, the co-cultivation of yeasts with microalgae and cyanobacteria took place in a multicultivator with gradual increase of selected macroelements – nitrogen, magnesium and phosphorus. The last part of the work was focused on the co-cultivation of yeasts and microalgae in a laboratory fermenter. The best effect on the production of total biomass was the increased magnesium content and high nitrogen content in the basic medium. The best concentrations of carotenoids were achieved due to the double nitrogen and phosphorus content together. Chlorophyll production was significantly lower compared to carotenoids.
|
|
|
Cocultivation of yeasts and microalgae to produce enriched biomass
Bradáčová, Lenka ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The diploma thesis is focused on the influence of biological stress formed by co-cultivation of heterotrophic (yeasts) and autotrophic (microalgae and cyanobacteria) organisms on the production of enriched biomass. The monitored groups of substances include carotenoids (-carotene, lutein, lycopene, astaxanthin, torularhodin), chlorophylls A and B, ergosterol and ubiquinone. Further, production of lipids was analyzed in the terms of fatty acid profile and lipid content in biomass. In the first part of the work, the yeast biomass production was investigated using different nitrogen sources. Glycerol was used as a carbon source in all parts of the work. Subsequently, the co-cultivation of yeasts with microalgae and cyanobacteria took place in a multicultivator with gradual increase of selected macroelements – nitrogen, magnesium and phosphorus. The last part of the work was focused on the co-cultivation of yeasts and microalgae in a laboratory fermenter. The best effect on the production of total biomass was the increased magnesium content and high nitrogen content in the basic medium. The best concentrations of carotenoids were achieved due to the double nitrogen and phosphorus content together. Chlorophyll production was significantly lower compared to carotenoids.
|
|
|
Supervisory and controlling activities of the public prosecutor against a background of the historical developments since 1948 up to the present day
Rokoská, Ilona ; Kuba, Jaroslav (advisor) ; Bradáčová, Lenka (referee)
The thesis deals with the supervisory and controlling activities of the public prosecutor against a background of the historical developments since 1948 up to the present day in Czechoslovakia and its successor states. It focuses on the key changes that have taken place within the public prosecution institutions and depicts the specific forms these bodies have taken along the years. Subsequently it points out the most recent forms of the public prosecution authorities performing in the Czech and Slovak republics and compares them. The thesis marginally looks at the discussions accompanying the adoption of a new public prosecution law and it maps their course so far. While compiling the thesis, analytical, historical and comparative methods were being used, with the thesis relying prevailingly on books, legal regulations and technical papers as sources of information. Having processed all the background materials and having carried out a subsequent comparison within the framework of the historical developments since 1948 along with a comparison of the Czech and Slovak republics, I have eventually arrived at a conclusion that the notions of supervising and controlling are not interchangeable and that the goal of the thesis, which consisted in their delimitation and mutual differentiation in the first place, has been reached.
|
|
| |
guest ::
RSS feed.