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Fabrication and properties of photocatalyst layers with controlled porosity
Blecha, Tomáš ; Gemeiner, Pavol (referee) ; Dzik, Petr (advisor)
This bachelor thesis deals with fabrication of photocatalyst layers with controlled porosity and investigation of their properties. Polyvinyl pyrrolidone, polyethylene glycol and polyvinyl alcohol were used as templating agents. Prepared compositions were deposited on FTO glass using a drop-casting method and annealed at 450 °C. The structure of thin films was examined using optical and electron microscopy. The thin films were subsequently studied by photoelectrochemical measurements. Most resulting compositions showed relatively good electrical and optical properties, hinting at a potential for future use in practice.
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Fabrication and properties of photocatalyst layers with controlled porosity
Blecha, Tomáš ; Gemeiner, Pavol (referee) ; Dzik, Petr (advisor)
This bachelor thesis deals with fabrication of photocatalyst layers with controlled porosity and investigation of their properties. Polyvinyl pyrrolidone, polyethylene glycol and polyvinyl alcohol were used as templating agents. Prepared compositions were deposited on FTO glass using a drop-casting method and annealed at 450 °C. The structure of thin films was examined using optical and electron microscopy. The thin films were subsequently studied by photoelectrochemical measurements. Most resulting compositions showed relatively good electrical and optical properties, hinting at a potential for future use in practice.
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Methodology of non-invasive survey of library units
Vávrová, Petra ; Neoralová, Jitka ; Novotná, Dana ; Součková, Magda ; Kazanskii, Andrei ; Blecha, Tomáš ; Popelková, Daniela ; Kohoutová, Kristina ; Kocour, Vladimír
Cílem předkládané metodiky je představení vybraných technologií a postupů pro průzkum knihovních dokumentů, u kterých je předpokládán výskyt sekundárně použitých textových nosičů (tzv. zlomků), historicky cenných fragmentů poškozených nebo odstraněných textů a provenienčních znaků, vnitřních defektů knižní vazby nebo napadení hmyzem.
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The Study of metabolism of dihydromyricetin
Blecha, Tomáš ; Moserová, Michaela (advisor) ; Koblihová, Jitka (referee)
More attention has recently been paid to the chemopreventive compounds contained in food of plant origin. A large group of chemopreventive compounds are represented by flavonoids contained in many dietary supplements. One of the representatives of this group is dihydromyricetin, which is attributed to positive impact on human organism, in particular by its antioxidant, anti-inflammatory and hepatoprotective effects. Some flavonoids can act as modulators of enzymes of phase I and phase II metabolism of xenobiotics biotransformation by their inhibition or induction which can causean interference with endogenous metabolism. In first part of this present work, the effect of selected flavonoid compounds, such as biochanin A, dihydromyricetin, myricetin and their combinations on the gene expression of cytochrome P450 2E1 (CYP2E1) and sulfotransferases 1A1 (SULT1A1) in liver and small intestine of laboratory rats was studied. The small intestine was divided into three parts namely a proximal, middle and distal. At first, isolation of total RNA was made followed by conversion into cDNA using random hexamers as primers. By real-time PCR a significant decrease in gene expression of both enzymes in liver was found. The highest induction of gene expression was observed for both enzymes in proximal part of...
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Study of effect of carcinogenic benzo[a]pyrene on cytochromes P450 and cytochrome b5 expression in organs of the rat
Blecha, Tomáš ; Moserová, Michaela (advisor) ; Dračínská, Helena (referee)
Polycyclic aromatic hydrocarbons are a large group of organic compounds which consist solely of carbon and hydrogen atoms, two or more fused aromatic rings. They represent group of persistent organic pollutants (POPs) present in all components and fields of the environment. Benzo[a]pyrene is characteristic compound of polycyclic aromatic hydrocarbons formed by five fused aromatic rings. Benzo[a]pyrene is procarcinogen with genotoxic effects, which is metabolically activated by variety of enzyme systems such as cytochrome P450 and epoxide hydrolase to reactive metabolite of BaP-7,8-diol-9,10-epoxide (BPDE) and 9-hydroxy-4,5-epoxy-BaP. These reactive metabolites can form covalent DNA adducts. In the present work, we studied the influence of carcinogenic benzo[a]pyrene on the expression of cytochrome P450 (CYP) 1A1, 1A2 and cytochrome b5 in livers, kidneys and lungs of laboratory rats. Total RNA was isolated and afterwards converted into cDNA with the participation of random primers. Using polymerase chain reaction in real time (RT-PCR) the relatively gene expression of CYP1A1, CYP1A2 and cytochrome b5 was quantified in the organs of rats treated with BaP and control (untreated) animals to a reference gene (β-actin). It was found that benzo[a]pyrene significantly increases expression of CYP1A1 and...
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