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Final report about research in Sokolov post mining sites in 2023 based on agreement between ENKI ops and Institute of Soil Biology and Biogeochemistry BC CAS.
Frouz, Jan ; Lišková, K. ; Bartuška, Martin ; Badraghi, A. ; Mudrák, O. ; Kučera, J. ; Montagnani, L. ; Bednářová, E. ; Šimáňová, D. ; Reinshmidová, E.
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology and biogeochemistry, BC CAS.
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Využití různých typů povrchově modifikovaných magnetických nanočástic pro izolaci a purifikaci bakteriální DNA/RNA
Bednářová, Eliška
In the submitted thesis "The use of different types of surface modified magnetic nanoparticles for isolation and purification of bacterial DNA/RNA", magnetic nanoparticles modified with tetraethoxysilane (TEOS) and (3-aminopropyl)triethoxysilane (APTES) were used. The aim was to optimize the nucleic acid isolation protocol using magnetic particles from both gram negative and gram positive bacteria in order to achieve the highest possible RNA yield. In the theoretical part of the thesis, the structure and composition of bacterial cells were summarized, especially the composition of the cell wall, which allows the bacteria to be divided into gram positive and gram negative. Furthermore, the different options for bacterial detection used in clinical practice were presented. Nucleic acid isolation is often an essential step in the detection of bacteria using molecular methods. A method based on magnetic particles is one of them, and the principle was explained here. The composition and functionalisation of the particles used for nucleic acid isolation have been described. In the practical part of the thesis, magnetic particles were characterized by several methods to specify their properties. Thus, the most suitable particles for nucleic acid isolations was selected. Next, the particles were used to optimize the procedure for nucleic acid isolation from cell lysate. The lysis buffer with 30% Triton X-100 detergent was determined to be the most effective and similar results were obtained with the 30% EcoSurf detergent. Subsequently, a method for efficient bacterial cell lysis was optimalized. Several methods were used and the best results were achieved by mechanical homogenization with 0.1 mm zirconia beads in combination with lysis buffer with 1% EcoSurf detergent. RNA yields from isolations by magnetic particles were compared with those from column isolations using a commercial isolation kit. Approximately 350 ng/µl of RNA yield was achieved for S. aureus using the column method, while 70 ng/µl less was achieved using magnetic particles. RNA yields were significantly higher than those using custom buffers, therefore, further optimization of the isolation protocol and composition of custom buffers in nucleic acid isolation would be advisable. Magnetic particles with both TEOS and APTES modifications have great potential for use in the isolation of nucleic acids from bacteria.
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Final report about research in Sokolov post mining sites in 2022 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Badraghi, A. ; Král, K. ; Reitschmiedová, E. ; Šálek, V. ; Kotápišová, Marta ; Novotná, B. ; Šimáňová, D. ; Kučera, J. ; Bednářová, E. ; Hublová, L. ; Irsah, S. ; Lačná, K. ; Kaneda, S. ; Zedníková, Petra
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Final report about research in Sokolov post mining sites in 2021 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Oppong, J.C. ; Lišková, K. ; Houška, J. ; Červenka, J. ; Kučera, J. ; Bednářová, E. ; Reitschmiedová, E. ; Dvorščík, P. ; Mudrák, O. ; Šimáňová, D. ; Woś, B. ; Karimi Nezhad, Mohammad Tashin ; Mustafa, Adnan ; Pietrzykowski, M. ; Kopecký, T. ; Hovorková, M. ; Čápová, K.
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Synthesis and application of new bipyridine ligands
Bednářová, Eva ; Kotora, Martin (advisor) ; Cibulka, Radek (referee) ; Michelet, Véronique (referee)
2,2'-Bipyridines and their appropriate N,N'-dioxides form a significant class of heteroaromatic compounds, which has found application in various fields of chemistry and predominantly in asymmetric catalysis. One of the most powerful methods for their synthesis is cocyclotrimerization of alkynes with nitriles. A new variant of cyclotrimerization reaction - cocyclotrimerization of halodiynes with nitriles, which results in the formation of 2- and 3-halopyridines, has been developed. The reaction was studied on a wide range of substrates providing the pyridine products in good isolated yields. Formation of an unexpected product of halogen exchange reaction was observed during the course of the study and its origin was elucidated by experimental studies. The prepared 2-halopyridines were used as starting materials for syntheses of new chiral 2,2'-bipyridine ligands. The crucial step of their synthesis turned out to be the reductive dimerization of 2-halopyridines to the corresponding 2,2'-bipyridines. Application of the formed bipyridine ligands was then tested in various metal-catalyzed asymmetric reactions, namely Mukaiyama aldol reaction, hydroxymethylation, conjugate addition, C-H activation of indole and desymmetrization of meso-epoxides, in which one of the bipyridine ligands showed...
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Synthesis and application of new bipyridine ligands
Bednářová, Eva
2,2'-Bipyridines and their appropriate N,N'-dioxides form a significant class of heteroaromatic compounds, which has found application in various fields of chemistry and predominantly in asymmetric catalysis. One of the most powerful methods for their synthesis is cocyclotrimerization of alkynes with nitriles. A new variant of cyclotrimerization reaction - cocyclotrimerization of halodiynes with nitriles, which results in the formation of 2- and 3-halopyridines, has been developed. The reaction was studied on a wide range of substrates providing the pyridine products in good isolated yields. Formation of an unexpected product of halogen exchange reaction was observed during the course of the study and its origin was elucidated by experimental studies. The prepared 2-halopyridines were used as starting materials for syntheses of new chiral 2,2'-bipyridine ligands. The crucial step of their synthesis turned out to be the reductive dimerization of 2-halopyridines to the corresponding 2,2'-bipyridines. Application of the formed bipyridine ligands was then tested in various metal-catalyzed asymmetric reactions, namely Mukaiyama aldol reaction, hydroxymethylation, conjugate addition, C-H activation of indole and desymmetrization of meso-epoxides, in which one of the bipyridine ligands showed...
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Final report about research in Sokolov post mining sites in 2020 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Lišková, K. ; Angst, Gerrit ; Sun, Daquan ; Kučera, J. ; Bednářová, E. ; Spurná, V. ; Jílková, Veronika ; Heděnec, P. ; Ćápová, K. ; Müllerová, V. ; Irshach, S.
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Final report about research in Sokolov post mining sites in 2019 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Kopecký, T. ; Kučera, J. ; Bednářová, E. ; Krůček, M. ; Král, K. ; Kotápišová, M. ; Moradi, J. ; Lin, Qiang ; Dini-Andreote, F. ; Meador, Travis Blake ; Angel, Roey ; Harantová, L. ; Heděnec, P. ; Li, Lingjuan ; Baldrian, P. ; Angst, Šárka
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Synthesis and application of new bipyridine ligands
Bednářová, Eva
2,2'-Bipyridines and their appropriate N,N'-dioxides form a significant class of heteroaromatic compounds, which has found application in various fields of chemistry and predominantly in asymmetric catalysis. One of the most powerful methods for their synthesis is cocyclotrimerization of alkynes with nitriles. A new variant of cyclotrimerization reaction - cocyclotrimerization of halodiynes with nitriles, which results in the formation of 2- and 3-halopyridines, has been developed. The reaction was studied on a wide range of substrates providing the pyridine products in good isolated yields. Formation of an unexpected product of halogen exchange reaction was observed during the course of the study and its origin was elucidated by experimental studies. The prepared 2-halopyridines were used as starting materials for syntheses of new chiral 2,2'-bipyridine ligands. The crucial step of their synthesis turned out to be the reductive dimerization of 2-halopyridines to the corresponding 2,2'-bipyridines. Application of the formed bipyridine ligands was then tested in various metal-catalyzed asymmetric reactions, namely Mukaiyama aldol reaction, hydroxymethylation, conjugate addition, C-H activation of indole and desymmetrization of meso-epoxides, in which one of the bipyridine ligands showed...
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Final report about research in Sokolov post mining sites in 2018 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Mudrák, Ondřej ; Sun, Daquan ; Müllerová, V. ; Ardestani, Masoud Mortazavi ; Zeníková, P. ; Veselá, H. ; Veselá, M. ; Cejpek, J. ; Dvorščík, P. ; Hnilička, F. ; Jačka, L. ; Walmsley, A. ; Kučera, J. ; Bednářová, E. ; Buchbauerová, L. ; Reinshmitová, E. ; Vicentini, Fabio ; Baldrian, Petr ; Lin, Qiang
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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