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Analysis of secondary metabolites from Streptomyces by Raman spectroscopy in vivo
Mikulová, Adéla ; Petříčková, K. ; Bobek, J. ; Chroňáková, Alica ; Samek, Ota ; Pilát, Zdeněk
The work deals with the study of Raman spectra of secondary metabolites of bacteria of the genus Streptomyces, especially polyene antifungal agents. Purified isolates and commercial standards of these compounds were used to obtain reference spectra from which spectral markers characterizing specific details of the chemical structure were derived. Verification of the observed markers was performed using in silico Raman spectra predictions. Subsequently, these findings were used for the spectroscopic characterization of the composition of secondary metabolites in vivo, directly in bacteria growing on a solid medium. The developed methodologies will help to optimize the conditions for the biotechnological production of antibiotics.
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Study of the limnological components of the ecosytem of Lake Medard in 2022
Peterka, Jiří ; Hejzlar, Josef ; Nedoma, Jiří ; Znachor, Petr ; Seďa, Jaromír ; Vejřík, Lukáš
The report summarizes the results of research into the limnological and biological components of the ecosystem of the newly created Medard post-mining lake. The aim of the presented report is to summarize the results achieved for the year 2022 and recommendations for further monitoring. The year 2022 was the fifth year after the lake was completely filled, when water from the Ohře River was no longer added. Only small tributaries flowed into the lake from its own catchment area, and at the same time, water from the lake drained through seeps, which are apparently drained into the nearby rivers, the Ohře and the Svatava. The lake ecosystem reached the final state of its initial phase in terms of hydrological regime and nutrient load. This condition is characterized by very little water exchange and a low nutrient level with low productivity of plankton communities. There is the development of submerged macrovegetation in littoral zone and a specific seasonal change in chemistry and biological parameters. However, the aquatic ecosystem is still far from stabilized and the biological communities of the littoral zone (littoral) and open water (pelagial), including the fish community, still show significant interannual dynamics.
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Complex survey of the fish community of the Most Lake in 2023
Peterka, Jiří
The report summarizes the results of a comprehensive survey of the fish population of Lake Most in 2022. The goal of the ichthyological survey of the lake was to map the state and development of the fish population, with an emphasis on estimating the abundance and biomass of individual fish species, estimating the risks of further development of the fish population and recommending further management of the lake. A summary of the achieved results and a proposal for recommendations for further monitoring are the contents of the presented report.
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Complex survey of the fish community of the Milada Lake in 2022
Peterka, Jiří
The report summarizes the results of a comprehensive survey of the fish population of Lake Milada in 2022. The goal of the ichthyological survey of the lake was to map the state and development of the fish population, with an emphasis on estimating the abundance and biomass of individual fish species, on estimating the risks of further development of the fish population and recommending further management of the lake. A summary of the achieved results and a proposal for recommendations for further monitoring are the contents of the presented report.
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