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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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Networks of Actors of Regional Development in Local Action Group Pošumaví
Petříková, Lenka ; Perlín, Radim (advisor) ; Maříková, Pavlína (referee)
The thesis deals with actors'network problems of regional development in the Local Action Group Pošumaví (LAG). The aim is to assess the functioning of networks within the Local Action Group Pošumaví focusing on the existence of networks, their activity and permeability and the way and intensity of involvement of the regional development actors in those networks. In the context of the institutional framework conditions of the local action groups a questionnaire survey together with guided interviews with actors LAG were analyzed. The results show that in the current programming period, the Local Action Group Pošumaví formed the network, but the cooperation is not very intensive. On the other hand, part of the actors of the regional development want to be more involved in LAG in the future, which can lead to extending and deepening of the existing cooperation.
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Evaluation of the European Cohesion Policy in Selected Region
Podzimková, Ivana ; Tomšík, Karel (advisor) ; Pavel, Pavel (referee)
The diploma's thesis is aiming to evaluate European policy of social cohesion and its growth options in the selected region. The evaluation is connected to the Elbe cycle route and it is based on analyses.
The theoretical part of the thesis introduces the subject of European policy of social cohesion while defining its legislation and framework of the legislation. It also defines the embodiment of regional politics within legislation of the local government i.e. the Czech Republic.
Analyses of the development of the selected project i.e. the Elbe cycle route and its impact on Hradec Kralove district will be dealt with in the practical part of the thesis. Business results of entities participating on the Elbe cycle route project will be gathered and used in order to evaluate selected indicators. Final assessment will be based on benchmarking. Method of comparison, analyses and syntheses will be used to evaluate gathered data. Applicable data will be gathered by research of related literature. Information will be also provided by the executive entities connected to the project, regional websites focussed on cycle tourism and by the town of Smiřice. Data gathered by the entities that cooperate on supervision of the Elbe cycle route project will be used for the statistic assessment.
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Political aspects of local goverment. Elections and electoral behavior of citizens in the town of Nový Bydžov
Bieliková, Eva ; Kopřiva, Radek (advisor) ; Lenka, Lenka (referee)
The thesis addresses the issue of elections and electoral behavior of citizens in the town of Nový Bydžov. It is focused on assessment of factors that influence voting behavior of citizens in the town of Nový Bydžov. It describes the evolution of electoral behavior from 1994 to 2014 and verification of the applicability of the theory of electoral behavior for explanation of the electoral decision-making of the electorate in Nový Bydžov. The theoretical part addresses (describes) theoretical definition of areas that relate to the topic of the thesis. The practical part is a case study of Nový Bydžov. Subject of study is the town of Nový Bydžov, objects of study are the voters participating in the elections to the council of this city. To achieve the set goals (objectives) is recovered content of documents study (written records), a questionnaire survey, comparative methods and synthesis.
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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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Demography and Dispersal Ability of a Threatened Saproxylic Beetle: A Mark- Recapture Study of the Rosalia Longicorn (Rosalia alpina)
DRAG, Lukáš
This thesis focuses on demography and mobility of an endangered and strictly protected icon of European saproxylic biodiversity, the Rosalia longicorn beetle (Rosalia alpina). Using the mark-recapture method we estimated the size of the population, adult longevity, and dispersal ability of the Rosalia longicorn on three hills in the Ralska Upland, Czech Republic. Furthermore, we assessed the distribution pattern of another 15 populations inhabiting small patches of old beech forest in this area. Factors affecting local survival and the species conservation are discussed.
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Provision of alternative refuse collection for a real estate surrounded by foreign property
Kvapil, Radek ; Štindl, Michal (referee) ; Klika, Pavel (advisor)
The thesis deals with possible ways of drainage and wastewater disposal. The issue of wastewater discharge to the environment has been closely monitored in recent years, and there is an apparent effort to improve the current situation.The dissertation discusses and compares three selected options – access to improved sanitation, building reservoirs and domestic wastewater treatment plant.The final section is dedicated to a comparison of acquisition and operating costs over time and to subsequent evaluation of the most suitable option. For the sake of clarity and assessment of potential solutions, the locality in the administrative area Vysoké Mýto - Domoradice has been selected as a model. The local authorities have been designing a public storm sewer, and given that it is surrounded by land, this issue will need to be addressed.
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Regulation of access electronic communications networks in the Czech Republic and its impact on the industry development
Sekaninová, Renáta ; Ševčík, Miroslav (advisor) ; Munzi, Tomáš (referee)
The thesis deals with the asymmetric ex-ante regulation applied in the electronic com-munications sector in order to strengthen the competitive ability of alternative operators at the expense of incumbent operators' competitiveness with the aim of reinforcing effective competition in the electronic communications markets. The author is concer-ned with the regulatory instrument entailing an obligation on the fixed incumbent opera-tors to open their access networks to other operators. This is called Local Loop Unbund-ling (LLU). The thesis assesses regulatory impacts on the Czech electronic communi-cations market. The market development in the Czech Republic certified the negative regulatory effect on the fixed alternative operators'motivation to build their own access infrastructure. The author tries to find the dismotivating elements of the present-day LLU regulatory form by means of the Czech LLU market analysis. The author adverts to the undesirable regulatory effect on both alternative operators'and incumbent opera-tors'investment motivation. The thesis offers reasoning why the pro-competitive regula-tory policy (inhibiting investments necessary for the sector development) is not appro-priate.
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Sharing Local Information for Faster Scanning-Window Object Detection
Hradiš, Michal ; Kälviäinen, Heikki (referee) ; Matas, Jiří (referee) ; Zemčík, Pavel (advisor)
Cílem této dizertační prace je vylepšit existující detektory objektů pomocí sdílení informace a výpočtů mezi blízkými pozicemi v obraze. Navrhuje dvě metody, které jsou založené na Waldově sekvenčním testu poměrem pravděpodobností a algoritmu WaldBoost. První z nich, Early non-Maxima Suppression , přesunuje rozhodování o potlačení nemaximálních pozic ze závěrečné fáze do fáze vyhodnocování detektoru, čímž zamezuje zbytečným výpočtům detektoru v nemaximálních pozicích. Metoda neighborhood suppression doplňuje existující detektory o schopnost zavrhnout okolní pozice v obraze. Navržené metody je možné aplikovat na širokou škálu detektorů. Vyhodnocení obou metod dokazují jejich výrazně vyšší efektivitu v porovnání s detektory, které vyhodnocují jednotlivé pozice obrazu zvlášť. Dizertace navíc prezentuje výsledky rozsáhlých experimentů, jejichž cílem bylo vyhodnotit vlastnosti běžných obrazových příznaků v několika detekčních úlohách a situacích.
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