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The development of Leishmania in the insect vector of the genus Phlebotomus
Kvapilová, Kateřina ; Volf, Petr (advisor) ; Bilej, Martin (referee)
The work was focused on the study of various aspects of development of Leishmania in vectors of genus Phlebotomus and can be divided into two main parts. In the first part, we studied the competition of Leishmania major and L. turanica during their development in the sand flies who are natural vectors of L. major using the experimental co-infections of fluorescently marked promastigots of these two species. While both leishmania species developed similar in the intestine of Phlebotomus papatasi, L. turanica prevailed in P. duboscqi in the late stages of infection. The fluorescent marking of Leishmania should allow us also to study possible genetic exchange between species at different stages of Leishmania infection (2nd, 9th and 14th day after the infective feeding). Using the flow cytometry (FACS) we have repeatedly identified dozens of objects emitting red and green signals in the intestinal homogenates of co-infected sand flies, however further analysis with confocal microscope disproved these objects as the hybrid promastigots of Leishmania. In the second part of this thesis we investigated the role of L. major genes HASP and SHERP, which lies on the locus LmcDNA16 and are expressed exclusively in metacyclics. We used mutant lines KO (lacking the locus LmcDNA16) and HASPB (KO line with gene...
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Employee motivation and satisfaction in production company
Olszowá, Zuzana ; Matysová, Kamila (advisor) ; Chylíková, Hana (referee)
The main subject of the thesis is employee motivation and satisfaction. The aim of this thesis is to analyze the employee motivation and work satisfaction of a production company, identify areas of dissatisfaction and provide recommendations for future improvements. The study also aims to emphasize the relationship between employee motivation and work satisfaction and the level of their performance, loyalty, absenteeism, fluctuation as well as the correllation between employee motivation and satisfaction and the overall organizational performance. A brief introduction is followed by the theoretical framework of the thesis which includes such concepts as motivation, stimulation, work motivation, job satisfaction or motivation profile, explains and focuses on basic theories of motivation and factors of employee satisfaction. The empirical part of the thesis introduces the human resources management in the production company and the questionnaire investigation conducted among the employees of the production company. The questionnaire icludes parts where the questions about satisfaction of the employee with the selected factors influencing employee motivation and satisfaction were asked. This section also includes conclusions and recommendations for future employee motivation and satisfaction improvements.
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Film induced tourism
Čepková, Petra ; Abrhám, Josef (advisor) ; Kalábová, Markéta (referee)
The master thesis deals with new phenomenon in tourism film induced tourism. The main objective of the master thesis is to characterize film induced tourism which could be used as a tool to increase the attendance of the destination where the film was shot. Next main objective of the thesis is evaluation of film induced tourism in Europe, more precisely in Great Britain and Northern Ireland where the film induced tourism is at relatively advanced level. The master thesis is divided into the eight main chapters. The first part briefly defines term tourism, its importance and tips related to the place of realization. In the second chapter there is described management of destination, following with marketing direction in the fourth chapter. In the fifth part, there is definition of film-induced tourism, related history, supply, the opportunities of marketing promotion and typology of the film induced tourist. Next parts of thesis show significance and efficiency of film induced tourism, film induced tourism in Czech Republic and in the Europe where the main attention was focused on Great Britain and Northern Ireland. The last chapter refers to own research where the potential of film induced tourism was investigated by using quantitative and qualitative methods from respondents which were based in Czech Republic.
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International involvement in the peacebuilding process on Sri Lanka
Mojžíš, Michal ; Havlová, Radka (advisor) ; Burešová, Jana (referee)
The final months of Sri Lankan civil war, the 26 year military campaign between the Government of Sri Lanka and the insurgent separatist organization Liberation Tigers of Tamil Eelam, are marked by allegations of war crimes committed by both parties of the conflict, including attacks on civilians, summary executions of prisoners, enforced disappearances, restrictions on humanitarian assistance and recruitment of children. Since the attempts to put the Sri Lankan issue on the agenda of the United Nations Security Council failed on the unwillingness of China and Russia, the UN bodies in Geneva have taken a leading role in promoting the peacebuilding process on the island. The thesis aims to analyse the impact of the Geneva-based human rights bodies, in particular of the United Nations Human Rights Council, on the process of reconciliation in Sri Lanka, for which is crucial that the alleged violations of human rights in the last months of the civil war would be properly investigated and that the perpetrators would be held accountable. In order to put the activity of the UN Human Rights Council into broader context, the paper will examine the political development in Sri Lanka since the end of the civil war in 2009 as well as the failed efforts of Sri Lankan governments to launch the process of reconciliation.
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Possibilities of Big Data use for Competitive Intelligence
Verníček, Marek ; Molnár, Zdeněk (advisor) ; Šperková, Lucie (referee)
The main purpose of this thesis is to investigate the use of Big Data for the methods and procedures of Competitive Intelligence. Among the goals of the work is a toolkit for small and large businesses which is supposed to support their work with the whole process of Big Data work. Another goal is to design an effective solution of processing Big Data to gain a competitive advantage in business. The theoretical part of the work processes available scientific literature in the Czech Republic and abroad as well as describes the current state of Competitive Intelligence, and Big Data as one of its possible sources. Subsequently, the work deals with the characteristics of Big Data, the differences from working with common data, the need for a thorough preparation and Big Data applicability for the methods of Competitive Intelligence. The practical part is focused on analysis of Big Data tools available in the market with regard to the whole process from data collection to the analysis report preparation and integration of the entire solution into an automated state. The outcome of this part is the Big Data software toolkit for small and large businesses based on their budget. The final part of the work is devoted to the classification of the most promising business areas, which can benefit from the use of Big Data the most in order to gain competitive advantages and proposes the most effective solution of working with Big Data. Among other benefits of this work are expansion of the range of resources for Competitive Intelligence and in-depth analysis of possibilities of Big Data usage, designed to help professionals make use of this hitherto untapped potential to improve market position, gain new customers and strengthen the existing user base.
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HYDROGENATION PROPERTIES OF BALL-MILLED Mg-Ti-C-Zr COMPOSITE
Král, Lubomír ; Čermák, Jiří ; Roupcová, Pavla
The hydrogen storage properties of ball-milled Mg-Ti-Zr-C composite (1.8 wt.% Ti, 1.9 wt.% Zr and 0.2 wt.% C) were investigated. It has been previously shown, that the addition of Ti, Zr and C improved its storage properties. This beneficial effect of additives upon hydrogen storage properties can be explained by catalysis by the particles rich in Ti or Zr located on the surface of Mg grains. They provide effective pathways for the hydrogen diffusion into the MgH2. The morphological and microstructural characteristics were investigated by scanning electron microscopy and by X-ray diffraction. The hydrogen sorption was measured by Sieverts method using Setaram PCT-Pro device. In this paper, sorption behaviour of the composite after ball-milling and after aging on the air was compared. The ball-milled composite adsorbed 3.5 wt.% H2 within 10 min at 623 K. However, hydrogen storage capacity of the composite aged on the air for 7 months remarkably decreased: The aged composite adsorbed within 10 min only 2 wt.% H2 at 623 K and the sorption capacity decreased from 4.7 wt.% H2 to 2.1 wt.% H2.
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Changes in structure and phase composition in the surface of tram rail
Švábenská, Eva ; Roupcová, Pavla ; Schneeweiss, Oldřich
We have investigated structure and phase composition of surface layer of tram rails after long time running and the results were compared with those obtained on the original part of material. Changes due to effects of severe plastic deformation together with thermal shocks by friction process were expected. The information about structure and phase composition was obtained by optical and scanning electron microscopy, X-Ray Powder Diffraction, Mössbauer Spectroscopy and Glow Discharge Emission Spectroscopy (GDOES) and this was completed by microhardness measurements. The results show that the surface layer in comparison with the original material exhibits important changes in grain structure, an increase in microhardness and high content of iron oxide and hydrooxides. According to the depth profile of the chemical composition measured by GDOES there is an increase in carbon content in the surface layer which can be effect of up-hill diffusion.
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Nursing care for woman at minor gynecological surgeries
KÁPLOVÁ, Veronika
Minor gynecological surgeries are defined as surgeries after which the patients are dismissed from hospital to home care within 12 to 24 hours. The purpose of pre-surgery care provided by a midwife is to ensure thorough somatic and mental preparation of the patient for the surgery. The patient is required to sign all necessary consents with the surgery, she shall be informed about the course of pre-surgery care, the surgery itself, post-surgery recovery and naturally also about all risks involved. The result of a consistent instruction provided by a midwife shall be a patient who is calm and mentally prepared for the surgery (Henková, 2010; Repková, 2006). The bachelor thesis includes a theoretical part and a practical part with research. The theoretical part focuses on minor gynecological surgeries and nursing care before and after the surgery. The objective of the bachelor thesis was to survey the level of awareness among women about minor gynecological surgeries. Two research questions were formulated to meet the objective: what the level of awareness about minor gynecological surgeries is among women and which method of information provision would be appreciated by women. The research part of the bachelor thesis employed the method of qualitative survey. The data were collected by means of semi-structured interviews. The surveyed set of respondents consisted of 8 women. The interviews were conducted from the beginning of February to the end of March 2014. The survey was expected to provide information about whether the women had been sufficiently informed about minor gynecological surgeries. For the category of pre-surgery period we investigated the following: What is the experience of women with previous nursing care? To what extent were the women informed by their local gynecologists and were they sufficiently informed in the hospital before the surgery? The results have shown that most of the respondents did not have any previous experience with nursing care. Two respondents said that they had previously undergone a minor surgery and that they were content with the nursing care. Results of the survey have shown some gaps in the form and quantity of information provided in medical offices of the local gynecologists. One half of the respondents were not content with the information provided in the medical offices. The information provided in the hospital before the surgery was assessed positively by five respondents out of eight and they appreciated particularly the personal and accommodating approach, professional conduct and empathy. For the category of post-surgery period we investigated how the respondents saw the approach of the nursing staff in the operating room. The results have shown that the respondents evaluated positively particularly the fact that the physician introduced himself/herself to the patient and provided information about the subsequent care. For the category of post-surgery care we also investigated subjective feelings of the respondents after the surgery, their awareness of the post-surgery regime at home and the form of information provision. Results of the research suggest that patients are highly perceptive, particularly when it comes to friendly gestures and words. The information about the home post-surgery regime was rated positively by one half of the respondents and negatively by the other half. On the other hand, they appreciated the personal and accommodating approach and professional conduct. Most of the respondents were content with the form of information provision. Some of them would have appreciated to have a leaflet or information brochure containing all important information. The theoretical part of the bachelor thesis can be as used as a study material for future students of midwifery at the Faculty of Health and Social Studies of the University of South Bohemia.
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PM2.5 Chemical Composition at the Rural Background Site Košetice.
Schwarz, Jaroslav ; Cusack, Michael ; Karban, Jindřich ; Chalupníčková, E. ; Havránek, Vladimír ; Smolík, Jiří ; Ždímal, Vladimír
Measurements of atmospheric aerosols at rural background areas are important because they are not influenced by the urban emissions, and are therefore better suited to investigate the influence of long-range transport and trends in concentrations of individual pollutants. Summary of the measurements of total concentration and the concentration of PM10 and PM2.5 components was carried out in the works Putaud et al. (2004, 2010). Although the later work included the measurement of more than 60 stations across Europe, the post-communist countries contained data on PM2.5 from only four locations (Prague, Debrecen, Hungary, and two rural locations). Although Košetice place where this study was made, is also discussed there, it was for only PM10 and total carbon (TC). Therefore, the aim of this study is to fill this information gap, and provide additional information regarding the PM2.5 fraction and composition at the Central European background station Košetice.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016110313110 - PDF
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