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Bioremediation of Creosote - contaminated Soil
BYSS, Marius
Bioremediation of creosote-contaminated soil was studied employing the methods of soil microbial biology and using new gas chromatography-mass spectrometry-mass spectrometry analytical approach. The changes of the soil microbial community under the polycyclic aromatic hydrocarbons (PAH) pollution impact were analyzed and described, as well as the changes during the bioremediation experiments. Laboratory-scale bioremediation experiments using the soil microbial community (consisted of bacterial inoculum capable of degrading PAH and of original soil microbial community) and employing ligninolytic fungi Pleurotus ostreatus and Irpex lacteus were conducted.
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Composing Data-driven Circuits Using Handshake in the Clock-Synchronous Domain
Sýkora, Jaroslav
We present a technique for modelling and synthesis of fine-grained data-driven circuits in the clock-synchronous hardware, such as the field programmable gate arrays (FPGA), called the Flow-Transfer Level (FTL). The distinguishing property of the FTL technique is that it does not rely on FIFO queues to handle flow synchronization between the components (called operators). The communication channels, called pipes, employ conceptually a two-state handshake protocol. The handshake behaviour of each operator is defined logically using dependency subgraphs that are symmetrical for producers and consumers. The original data-flow netlist of operators is transformed into a global control dependency graph. Cycles in dependency graphs are allowed as long as they do not constitute real data dependencies but only dependencies in promises of handshake completions. A method is given that recursively eliminates these cycles.
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The need for security and safety in connection with a planned surgery with the patient's perspective and nurses
BOHDALOVÁ, Jaroslava
A planned operation performance always affects patient´s needs for assurance and safety. It is important to provide necessary information connected with the planned operation performance itself so that the patient can feel assure and safe. The key role for the patient and her/his needs for assurance and safety is in general nurse´s hands because she is the closest person to the patient and can help to inspire her/his needs for assurance and safety with konfidence best through the aid of her knowledge and skills that she should have as well as through the aid of her attitudes. The target of the thesis was to find out how a planned operation performance affects patient´s needs for assurance and safety and how a general nurse inspires patient´s needs for assurance and safety connected with a planned operation performance with confidence. The following research questions were stated: How does a planned operation performance affect patient´s needs for assurance and safety? How does a general nurse affect patient´s needs for assurance and safety connected with a planned operation performance to inspire her/him with confidence? What is insufficient in filling patient´s needs for assurance and safety before a planned operation performance? How can a general nurse help a patient control her/his fear and state of anxiety related to a planned operation performance? The method qualitative research with the use of depth interview was used to figure out the investigation. The respondents were willing to record the interview which were verbally rewritten and then subjected to the contents analysis by the method paper and pencil. From the research it is clear that a planned operation performance affects patient´s needs for assurance and safety in a negative way disregarding to her/his previous experience. Patient´s worries and negative feelings can be eliminated by a general nurse who treats her/him as equal, taking up an individual approach, informing her/him about all performances and proceedings, having a calming and welcoming way of communication, using empathy and listening as well as trying to meet patient´s wishes according to her possibilities. The research reveals the advantage of patients´ readiness for planned operation performances. Patients can learn it in the framework of educational proceeding in the out-patients´ department at the same time when they learn that they are going to be operated. The research also shows that general nurses do not have enough knowledge in the sphere of therapeutical communication. The results indicate that it would be suitable to start educating patients as soon as it is known how their disease will be solved and treated in order to reduce the negative impact of surgical treatment to patients´ needs for assurance and safety. General nurses should complete their knowledge and skills to be able to help patients to cope with their difficult life-situation. On the basis of the research investigation and after the consultation with the senior nursing officer, the training course will be organized. The topic of the training course will concentrate on communication with patients before planned operation performances with a view to therapeutical communications and therapeutical interviews. Other discovered facts, for example, the lack of information provided to patients and possibilities regarding better patients´ readiness for planned operation performances will surely be solved by the hospital management.
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The effect of long-term fertilizers application on soil chemical properties, plant species composition and arbuscular mycorrhizal fungi of grasslands
Zemková, Lenka ; Pavlů, Vilém (advisor) ; Hakl, Josef (referee)
Fertilization and liming of grasslands have been used as an instrument improving hay production for centuries. For a long period of time, these practices not only sustained and increased hay yields, but also had negative effects on grassland ecosystems. Among others, they caused considerable changes in plant species composition leading in most cases to the reduction of species richness due to a lower capability of indigenous plants to compete with more productive pasture species. Furthermore these practices caused changes in soil and biomass chemical properties and shifts of soil microbial communities, including arbuscular mycorrhizal fungi.
The aim of this thesis was to analyze the responses of plant and mycorrhizal community and soil chemical properties in grassland ecosystems to long-term application of nitrogen, phosphorus, potassium, and calcium. Results of this work indicate that agricultural management practices aimed at maximization of hay production may, in the long run, significantly increased hay yield, reduce plant species richness, promote grasses but suppress herbs, legumes and arbuscular mycorrhizal fungi in the soil although the response to phosphorus varied among different arbuscular mycorrhizal fungal taxa. Long-term fertilization also significantly changed soil chemical properties and considerably reduced belowground carbon storage.
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Interspecific interactions in wetland communities in dependence on water regime: long-term test of stress-gradient hypothesis
Růžičková, Kateřina ; Douda, Jan (advisor) ; Alena, Alena (referee)
The Stress-gradient hypothesis predicates a change of interspecies relations from negative to positive according to the stress gradient. The basic assumption is that the presence of one species (the facilitator) makes the growth of other species easier. In this study was determined based on the 5years experiment, whether the interspecies interactions (i.e. the importance of facilitation and competition) changes according to the hydrological conditions. Three wetland species (Calamagrostis canescens, Carex elongata and Deschampsia cespitosa) were planted in experimental pots with the presence and absence of the dominant species (Carex elata). The hydrological gradient (= the stress gradient) was simulated by three types of hydrological conditions: dry, fluctuating and wet. The success of the species within the specific conditions was correlated with the fitness-related characteristics of the plants including the biomass, the number of ramets and the height of the plants. Following hypotheses were tested: i) at a low intensity of stress (with the wet treatment), the C. elata represents a competitor for other species. On the other hand, at a high intensity of stress (with the dry treatment), the C. elata represents a facilitator, making it easier to the other coexisting species to grow. This presumption was confirmed only with the D. cespitosa, which was more successful under dry treatment with the presence of a dominant species, which facilitated it. With the other two species the change of interspecific relations was not observed and the relationships remained negative. Furthermore the results showed, that in the dry treatment with the presence of a dominant species, there was a lower intensity of competition among the coexisting species. The experiment showed, that the interactions of particular species with dominant species can vary according to the stress gradient. The hydrological gradient also influences the character and intensity of interactions among the species.
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Diversity of insect communities of non-indigenous forests of black pine (Pinus nigra) in the western part of Central Bohemia
Tůmová, Klára ; Kadlec, Tomáš (advisor) ; Jakubíková, Lada (referee)
Due to the rapid intensification of agriculture, there were a bigger and more often habitat loss and their fragmentation. These led to the decreasing of the size of suitable habitats and their isolation. The isolation of landscape fragments is a basic geographical characteristic, which plays an important role in the ecological invasions. The invasions generally cause major economic damages and they affect the loss of biological diversity in the stands.
In this work, we focused on the effects of black pine (Pinus nigra). This wood plant has been occurred in the Czech Republic since the Tertiary period and disappeared by the subsequent disintegration of continents. The aim of this study is to determine the influence of black pine (Pinus nigra) on the invertebrates compared with the native stands of scots pine (Pinus sylvetris).
The experiment was conducted at 35 plots in Central Bohemia (18 plots of Scots pine, 17 plots of black pine). The invertebrates were captured by light traps. The total abundance and biomass of invertebrates were determined from the captured samples. From the processed data was not proven negative impact on non-native black pine to overall abundance and biomass of invertebrates. This fact is most likely due to the phylogenetic relationship of both pine species.
There were not found any differences in abundance and biomass of most herbivorous groups of invertebrates. However, in the black pine stands was reported a higher incidence of carnivorous species of invertebrates.
The stands of the black pine have no negative impact on number of invertebrates in the Czech Republic, actually there was demonstrated even higher affinity of carnivorous species of invertebrates to the black pine trees.
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The Most Holy Trinity as the Foundation of the Identity of Created Persons
HANKE JAROŠOVÁ, Světla
According to Scripture God created human beings "in our image, after our likeness". The likeness used to be conceived in terms of the rational nature human beings possess. Modern thought has come to conceive of the likeness rather in terms of being person, who receives her identity in relationships from others and at the same time mediates the others´ identity to them. The author presents the historical development of the concept of person as well as selected contemporary approaches, which she evaluates in light of the personal life of the Triune God as it is witnessed in Scripture and incessantly communicated in the liturgy of the church. She also presents ways of conceiving of created personhood she has attained in this light. Created persons are created in order to receive divine self-communication and to be able to participate in it, which they can in dependence on the one, who is the true revelator of God, as participation in his revelatory activity. By accepting their dependence, they become capable of acting freely through themselves and fulfill the task entrusted to them by creation.
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Monitoring and protection of habitats nature trail Pustý rybník (Dymokury district)
Nouzovský, Petr ; Česká, Jana (advisor) ; Dana, Dana (referee)
The aim of the thesis was to perform mycological survey of the site with a focus on the incidence and inventory of rare and endangered species of macromycetes. They were also assessed anthropogenic influences that affect the value of the territory and selected habitats. It was the first comprehensive mycological site survey, which was conducted from 2011 to 2015. The Trail Pustý pond near Dymokury is a relic of medieval basic hornbeam with almost unscrupulous management. From the natural communities Carpinion the area include Quercus petraea, Quercus Robur, Carpinus betulus, Tilia cordata and Acer Campestre. Natural forest communities Carpinion provides a very strong and long-lasting mycorrhiza. Minority There exists also a natural Crataegus monogyna, Quercus petraea, Fraxinus excelsior and Alnus glutinosa. Unoriginal there is only a pioneer Betula pendula and before about 60-years ago planted vegetation Pseudotsuga menziessii. The result of mycological research, which began in spring 2011 and it was completed 31. 12. 2015 has been detected and reported the occurrence of 294 kinds of macromycetes. Of this total were found and documented 39 rare and endangered fungi. Of macromycetes, which are not protected, found and determined 27 Ascomycetes and 267 Basidiomycetes. Of law protected species in the study area has been detected in the following species: Boletus fechtneri (§1), Xerocomus moravicus (§1), Tuber aestivum (§1), Volvariella caesiotincta (§2), Otidea concinna (§2) and Russula alnetorum (§ 3), of the species were categorized into the Red list of Czech Republic fungi is one of the most important findings species: Boletus fuscoroseus (CR), Boletus rhodopurpureus (CR), Boletus satanas (NT) Rubinoboletus rubinus (NT), Hygrophorus persoonii (CR), Lactarius lacunarum (NT), Lactarius pterosporus (NT), Lactarius zonarius (VU), Amanita echinocephala (EN). In the second part the focus is on the evaluation of anthropogenic pressures and the overall management at the site, as well as to natural succession. Majority is focused on the aftercare of land depending on the occurrence of rare and protected species of fungi and plants, and to assess the monitored habitat at the site. Given the interesting transition boundaries in an otherwise coherent natural habitat, where they were found other rare species of fungi have been to several locations are measured and recorded pH of the soil. Photographic documentation, which is incorporated by reference and inventory taxa, which is listed in the results, it can be used as an opportunity to re-create the old and dark educational boards on the surveyed area, as well as may be used to create small-scale protected area. Exsiccata have so far been kept as a resource in the private herbaria author. This thesis summarizes five years mycological study of the site in question strictly and justifies the use of the most appropriate management with regard to the occurrence of rare and protected species of fungi.
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The effects of neighbours in plant communities: mathematical and experimental approaches
FIBICH, Pavel
The effect of plant neighbourhood was investigating for hemiparasitic plants, productivity and diversity effects, constant final yield and recruits establishment. The research was based on a wide range of methods including the system of differential equations for population dynamic, individual based model with field of neighbourhood approach, glasshouse experiment and field experiment with gaps in the wet meadow.
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