|
Robust Approach for Environmental and Health Practice: Novel Paradigm for Data Treatment and Analysis
Wagner, Zdeněk ; Ocelka, T. ; Pavliska, L.
Knowledge of concentration of atmospheric aerosol particles may provide an important information in applications concerning human health. Aerosol studies reach into several fields of science. In the past decades it was shown that enhanced concentration of atmospheric aerosol lead to increased mortality on cardiopulmonary diseases and lung cancer. This work presents a robust method of estimation of particle size distribution based on mathematical gnostics. The algorithm may either be used as a manual tool for analyzing a small number of particle size distributions ex post or as a routine implemented in automatic monitoring devices for near real time processing of measured data and immediate online display on a web site. The method has sucessfully been implemented for near real time analysis of data measured by a scanning mobility particle sizer conformant to recommendations defined in the framework of the EUSAAR project.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22013071913360 - PDF
|
|
Ethicotherapy in Conception of Vladimír Vogeltanz
Laňková, Renata ; Klimešová, Anna (advisor) ; Sivek, Vojtěch (referee)
I wrote about ethicotheraphy because I'm interested in psychosomatic view of diseases in my work. In the first part I focused on a historical view of connection among body, soul and health. There are also chapters in which I introduced history of psychosomatic approach and ethicotheraphy generally with its methodical approach. In the second part I compared a relationships between the doctors with their patients and the ethicotherapists with their clients was compared, too. I described the ways of work in the ethicotheraphy and their clients and I mentioned a profit of disease. In the third part I wrote about a cancer in ethicotheraphy view. I wanted to show how difficult is to treat these disease all the other incidental difficulties. Powered by TCPDF (www.tcpdf.org)
|
|
The Public Attitude towards Preventive Oncology Examinations
KAULICHOVÁ, Markéta
The Public Attitude towards Preventive Oncology Examinations The object of this paper is to find out how laic and health professional public uses the potential of preventive oncology examinations and to uncover the barriers in use of these programs from the point of view of the laic and professional public. The public attitude towards preventive oncology examinations is more important these days as the incidence of oncology diseases is growing. Prevention is considered the principal in modern medicine, also from an economic point of view. The attitude of society towards its health is one of problematic public health areas, the attitude of self-responsibility and the underestimated meaning of primary prevention. Moreover, there is a psychological problem related to oncological diseases: the fear of positive findings and the fear of the disease itself which is traditionally connected with incurability, cruel pain and inevitable death. This notion is no longer true. A dictatorial doctor{\crq}s approach to patients in the past has been replaced with cooperative and more equal relation, where the patient takes part in decision making. However, doctors are too busy to find the proper way and proper extent of informing the patient or these doctors often underestimate the importance of a good way of giving this kind of information. This thesis presents current preventive care offered in the Czech Republic and it shows the negative bias towards cancer which is a stumbling block to a more successful fight against oncology diseases. This research was undertaken amongst laic and health public. Quantitative data collection was used in the form of questionnaires. The research file is made up of men and women over 18 years, with or without health occupation. The hypothesis was not proven that laic public use preventive oncology examinations more than health public. The laics use these programs less mostly due to lack of awareness about them. One of the most common obstacles in using preventive programs is the misunderstanding of prevention itself, when people contact a doctor only when they have problems. The results have also shown that laics are not satisfied with the activity of doctors in informing them and that laic public search for information is done less actively. This thesis can be used as a foundation for further discussion about problems of informing the public about preventive programs and for future education of health personnel within oncology matters.
|
|
Genetic Aspects of Sporadic Ovarian Cancer
Jančárková, Natalia ; Freitag, Pavel (advisor) ; Rokyta, Richard (referee) ; Kohoutová, Milada (referee)
58 9. SUMMARY Molecular biological parameters, including genetic alterations, present new and perspective direction in diagnostics, prediction of prognosis, monitoring and possible therapeutic approaches in oncological disease. The results presented have to do with the project, which is concentrated upon chromosomal rearrangements in ovarian and cervical cancer and their correlations with available parameters of both molecular biological and clinical characteristics. Sixty patients with ovarian cancer and twenty patients manifesting cervical cancer were included into the study. The histological type and grade, MIB-1 and p53 (using immunohistochemical method) were estimated by histopathologist. Both conventional karyotyping and molecular-cytogenetic methods (fluorescent in situ hybridization and comparative genomic hybridization) were applied to reveal chromosomal aberrations. The results were subjected to statistical evaluation, using analysis of variances and χ2 test. There were correlated parameters of quantitative and qualitative character - age, stage, histological type, grade, CA 125 before and after treatment, MIB-1, p53, surgical residuum, lymphadenectomy, response rate, chromosomal rearrangements. Analyses accomplished in ovarian cancer group revealed typical amplifications on chromosomes 1q, 3q and...
|
| |
| |
|
Methods for class prediction with high-dimensional gene expression data
Šilhavá, Jana ; Matula, Petr (referee) ; Železný, Filip (referee) ; Smrž, Pavel (advisor)
Dizertační práce se zabývá predikcí vysokodimenzionálních dat genových expresí. Množství dostupných genomických dat významně vzrostlo v průběhu posledního desetiletí. Kombinování dat genových expresí s dalšími daty nachází uplatnění v mnoha oblastech. Například v klinickém řízení rakoviny (clinical cancer management) může přispět k přesnějšímu určení prognózy nemocí. Hlavní část této dizertační práce je zaměřena na kombinování dat genových expresí a klinických dat. Používáme logistické regresní modely vytvořené prostřednictvím různých regularizačních technik. Generalizované lineární modely umožňují kombinování modelů s různou strukturou dat. V dizertační práci je ukázáno, že kombinování modelu dat genových expresí a klinických dat může vést ke zpřesnění výsledku predikce oproti vytvoření modelu pouze z dat genových expresí nebo klinických dat. Navrhované postupy přitom nejsou výpočetně náročné. Testování je provedeno nejprve se simulovanými datovými sadami v různých nastaveních a následně s~reálnými srovnávacími daty. Také se zde zabýváme určením přídavné hodnoty microarray dat. Dizertační práce obsahuje porovnání příznaků vybraných pomocí klasifikátoru genových expresí na pěti různých sadách dat týkajících se rakoviny prsu. Navrhujeme také postup výběru příznaků, který kombinuje data genových expresí a znalosti z genových ontologií.
|
| |
|
USE OF COUPLING REACTIONS IN THE SYNTHESIS OF POTENTIALLY BIOLOGICALLY ACTIVE COMPOUNDS
Hessler, Filip ; Kotora, Martin (advisor) ; Pour, Milan (referee)
This work consists of three parts, each dealing with the synthesis of different biologically active compound, using reactions mediated by transition metals. 1. Ferrocene conjugates with various types of natural or biologically active compounds have been studied intensively for their new interesting properties compared to the model compounds. It was decided to synthesize a new steroid containing ferrocene - ferrocenestrone, planned with regard to its possible activity against breast cancer cells. Although several conjugates of steroids with ferrocene have been prepared, the cyclopentadienyl ring has not been the integral part of the steroid skeleton in any of them. Ferrocenestrone, an analog of estrone, however contains ferrocene in place of the aromatic A-ring. The approach to the ferrocene-steroid framework construction was based on transition metal mediated reactions starting from a suitably substituted chiral ferrocene. The methods used were: zirconocene-mediated oxidative additions with successive alkylation sequences, palladium- catalyzed cross-coupling reactions, ruthenium-catalyzed skeletal rearrangements (enyne metathesis), palladium and iridium catalyzed hydrogenations etc. Also selective oxidation and subsequent borane reduction was used for the final change of skeletal configuration....
|
|
Topography of signaling molecules on the plasma membrane in the course of mast cell activation
Lebduška, Pavel ; Dráber, Petr (advisor) ; Šebo, Peter (referee) ; Benada, Oldřich (referee) ; Tučková, Ludmila (referee)
presented technique of plasma membrane sheet preparation from nonadherent cells may facilitate research in this field. It must be, however, mentioned that a plastic view ofsignal transduction across the plasma membrane can be achieved only by combination of various mutually complementary approaches. Conclusions Three techniques of lsoladon of plasmr m€mbrane sh$ts from nonadherent BMMC mast cells have bmn developed. one of them, based on edsorption ofl€ukocýes to glass suďace, turned out to be very promlsing md provided many scientifrc datr(article E). Actlvation of RBL m9st c€l|s by FGRI raeptor dimerintion led to increme of Grb2 adaptor content in the Plasma membrane. Ilowever' by contřast to the case of receptor mu|t|merintion, this Grb2 did not sign|ficantly colocallz€ w|th FERI' and' by |mmuno|rbeling of membrane she€ts, distribution of FC5RI wrs not d|stinguishabl€ from the disfibution on nonact|vrted ce|ls (article A). BMMC, In contrast to RBL cells, after multimerization of FaRI did not form larger aggregat€s ofthis r€c€ptor thrn nonact|vat€d cells did. FGRI muldmer|ation led to lts int€rna|iation of comparable intensity rnd overa|l dynemics ln BMMC end RBL cel|s' but loce| redistribut|on of FaRI fundamentďly differed betwcn these two c€|| wes (article E). Established mode| oflrrg€ (8pproxim8t€|y...
|
guest ::
